Overview

  • Product name

  • Description

    Rabbit polyclonal to MST
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human MST (N terminal) conjugated to keyhole limpet haemocyanin.
    Database link: NP_066949

  • Positive control

    • HeLa cell lysate; Mouse kidney tissue lysate.
  • General notes

    Protein previously labeled as MPST.

Properties

Applications

Our Abpromise guarantee covers the use of ab85377 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Predicted molecular weight: 33 kDa.
ELISA 1/1000.

Target

  • Function

    Transfer of a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. May have a role in cyanide degradation or in thiosulfate biosynthesis.
  • Sequence similarities

    Contains 2 rhodanese domains.
  • Domain

    The structure consists of 2 domains of very similar conformation, suggesting a common evolutionary origin. However, the sequences of the 2 domains are very different.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • 3 mercaptopyruvate sulfurtransferase antibody
    • 3-mercaptopyruvate sulfurtransferase antibody
    • Human liver rhodanese antibody
    • Mercaptopyruvate sulfurtransferase antibody
    • MGC24539 antibody
    • MPST antibody
    • MST antibody
    • THTM_HUMAN antibody
    • TST2 antibody
    see all

Images

  • Anti-MST antibody (ab85377) at 1/50 dilution + HeLa cell at 35 µg

    Predicted band size: 33 kDa
    Observed band size: 33 kDa
    Additional bands at: 35 kDa, 60 kDa, 70 kDa. We are unsure as to the identity of these extra bands.

  • Anti-MST antibody (ab85377) at 1/50 dilution + Mouse kidney tissue lysate at 35 µg

    Predicted band size: 33 kDa
    Observed band size: 30 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 20 kDa, 55 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:

  • Kondo K  et al. H2S protects against pressure overload-induced heart failure via upregulation of endothelial nitric oxide synthase. Circulation 127:1116-27 (2013). WB ; Mouse . Read more (PubMed: 23393010) »
See 1 Publication for this product

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