Key features and details
- Rabbit polyclonal to MST1/MST2
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MST1/MST2 antibody
See all MST1/MST2 primary antibodies
DescriptionRabbit polyclonal to MST1/MST2
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Turkey, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Tilapia, Orangutan, Xenopus tropicalis
Synthetic peptide derived from the C terminal region of Human MST2 using the numbering given in Swiss Prot entry Q13188. The epitope is highly conserved with MST1.
- Colon carcinoma, placenta, stomach adenocarcinoma.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab87322 in the following tested applications.
Likely to work with frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceThe human serine/threonine kinase, mammalian STE20 like kinase (MST), is considerably homologous to the budding yeast kinases, SPS1 and STE20, throughout their kinase domains. When stably expressed in HeLa cells, MST highly sensitizes the cells to death receptor mediated apoptosis by accelerating caspase 3 activation. These findings suggest that MST1 and MST2 play a role in apoptosis both upstream and downstream of caspase activation. MST1 is cleaved and activated by caspases during apoptosis and is capable of inducing apoptotic morphological changes such as chromatin condensation upon overexpression. Mammalian Sterile 20 Like 2 (MST2) is most similar to the previously identified MST1 protein kinase (78% identity, 88% similarity). Northern analysis indicates that MST2 mRNA is expressed at high levels in adult kidney, skeletal and placental tissues and at very low levels in adult heart, lung, liver and brain tissues. An in vitro kinase assay indicates that MST2 can phosphorylate an exogenous substrate, as well as itself, and phospho amino acid analysis indicates that it is a serine/threonine protein kinase.
Cellular localizationCytoplasm. Nucleus. Note: The caspase cleaved form cycles between the nucleus and cytoplasm.
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Detection of Human MST1/2 by immunohistochemistry on formalin-fixed, paraffin-embedded Human placenta tissue using ab87322 at a dilution of 1/250.
Detection: DAB staining.
Detection of Human MST1/2 by immunohistochemistry on formalin-fixed, paraffin-embedded Human colon carcinoma tissue, using ab87322 at a dilution of 1/250.
Detection: DAB staining.
ab87322 has been referenced in 2 publications.
- Karagianni AE et al. Transcriptional Response of Ovine Lung to Infection with Jaagsiekte Sheep Retrovirus. J Virol 93:N/A (2019). PubMed: 31434729
- Volckaert T et al. Fgf10-Hippo Epithelial-Mesenchymal Crosstalk Maintains and Recruits Lung Basal Stem Cells. Dev Cell 43:48-59.e5 (2017). PubMed: 29017029