Anti-MST3 antibody (ab155198)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-MST3 antibody
See all MST3 primary antibodies -
Description
Rabbit polyclonal to MST3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Xenopus tropicalis
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-247 of Human MST3
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Positive control
- 293T, A431, H1299, HeLaS3, HepG2, MOLT4, Raji whole cell lysate; HeLa cells
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General notes
Keep as a concentrated solution.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab155198 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 48 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Serine/threonine-protein kinase that acts on both serine and threonine residues and promotes apoptosis in response to stress stimuli and caspase activation. Mediates oxidative-stress-induced cell death by modulating phosphorylation of JNK1-JNK2 (MAPK8 and MAPK9), p38 (MAPK11, MAPK12, MAPK13 and MAPK14) during oxidative stress. Plays a role in a staurosporine-induced caspase-independent apoptotic pathway by regulating the nuclear translocation of AIFM1 and ENDOG and the DNase activity associated with ENDOG. Phosphorylates STK38L on 'Thr-442' and stimulates its kinase activity. Regulates cellular migration with alteration of PTPN12 activity and PXN phosphorylation: phosphorylates PTPN12 and inhibits its activity and may regulate PXN phosphorylation through PTPN12. May act as a key regulator of axon regeneration in the optic nerve and radial nerve. -
Tissue specificity
Isoform A is ubiquitous. Isoform B is expressed in brain with high expression in hippocampus and cerebral cortex. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsProteolytically processed by caspases during apoptosis. Proteolytic cleavage results in kinase activation, nuclear translocation of the truncated form (MST3/N) and the induction of apoptosis.
Isoform B is activated by phosphorylation by PKA. Oxidative stress induces phosphorylation. Activated by autophosphorylation at Thr-190 and phosphorylation at this site is essential for its function. Manganese, magnesium and cobalt-dependent autophosphorylation is mainly on threonine residues while zinc-dependent autophosphorylation is on both serine and threonine residues. -
Cellular localization
Cytoplasm. Nucleus. Membrane. The truncated form (MST3/N) translocates to the nucleus. Co-localizes with STK38L in the membrane. - Information by UniProt
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Database links
- Entrez Gene: 8428 Human
- Entrez Gene: 223255 Mouse
- Entrez Gene: 361092 Rat
- Omim: 604984 Human
- SwissProt: Q9Y6E0 Human
- SwissProt: Q99KH8 Mouse
- SwissProt: B0LT89 Rat
- Unigene: 508514 Human
see all -
Alternative names
- epididymis secretory protein Li 95 antibody
- HEL-S-95 antibody
- Mammalian STE20 like protein kinase 3 antibody
see all
Images
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Western blot - Anti-MST3 antibody (ab155198)All lanes : Anti-MST3 antibody (ab155198) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lane 4 : HeLaS3 whole cell lysate
Lane 5 : HepG2 whole cell lysate
Lane 6 : MOLT4 whole cell lysate
Lane 7 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 48 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-MST3 antibody (ab155198)Immunofluorescent analysis of methanol-fixed heLa cells labeling ANKRD32 with ab155198 at 1/100 dilution. Lower panel co-stained with Hoechst 33342.
References
This product has been referenced in:
- Shang Q et al. Effect of Plastrum Testudinis Extracts on the Proliferation and Osteogenic Differentiation of rBMSCs by Regulating p38 MAPK-Related Genes. Evid Based Complement Alternat Med 2019:6815620 (2019). Read more (PubMed: 30984279) »
- Jiang Y et al. Protein Kinase Serine/Threonine Kinase 24 Positively Regulates Interleukin 17-Induced Inflammation by Promoting IKK Complex Activation. Front Immunol 9:921 (2018). Read more (PubMed: 29760709) »
