Recombinant
RabMAb

Recombinant Anti-MST3 antibody [EP1468Y] (ab51137)

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Overview

  • Product name

    Anti-MST3 antibody [EP1468Y]
    See all MST3 primary antibodies

  • Description

    Rabbit monoclonal [EP1468Y] to MST3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IP, WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human MST3 aa 400-500 (C terminal). The exact sequence is proprietary.
    (Peptide available as ab198164)

  • Positive control

    • HeLa cells, human brain tissue.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab51137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP 1/20.
WB 1/5000. Detects a band of approximately 52 kDa (predicted molecular weight: 49 kDa).Can be blocked with Human MST3 peptide (ab198164).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.

Target

  • Function

    Serine/threonine-protein kinase that acts on both serine and threonine residues and promotes apoptosis in response to stress stimuli and caspase activation. Mediates oxidative-stress-induced cell death by modulating phosphorylation of JNK1-JNK2 (MAPK8 and MAPK9), p38 (MAPK11, MAPK12, MAPK13 and MAPK14) during oxidative stress. Plays a role in a staurosporine-induced caspase-independent apoptotic pathway by regulating the nuclear translocation of AIFM1 and ENDOG and the DNase activity associated with ENDOG. Phosphorylates STK38L on 'Thr-442' and stimulates its kinase activity. Regulates cellular migration with alteration of PTPN12 activity and PXN phosphorylation: phosphorylates PTPN12 and inhibits its activity and may regulate PXN phosphorylation through PTPN12. May act as a key regulator of axon regeneration in the optic nerve and radial nerve.

  • Tissue specificity

    Isoform A is ubiquitous. Isoform B is expressed in brain with high expression in hippocampus and cerebral cortex.

  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 protein kinase domain.

  • Post-translational
    modifications

    Proteolytically processed by caspases during apoptosis. Proteolytic cleavage results in kinase activation, nuclear translocation of the truncated form (MST3/N) and the induction of apoptosis.
    Isoform B is activated by phosphorylation by PKA. Oxidative stress induces phosphorylation. Activated by autophosphorylation at Thr-190 and phosphorylation at this site is essential for its function. Manganese, magnesium and cobalt-dependent autophosphorylation is mainly on threonine residues while zinc-dependent autophosphorylation is on both serine and threonine residues.

  • Cellular localization

    Cytoplasm. Nucleus. Membrane. The truncated form (MST3/N) translocates to the nucleus. Co-localizes with STK38L in the membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • epididymis secretory protein Li 95 antibody
    • HEL-S-95 antibody
    • Mammalian STE20 like protein kinase 3 antibody
    • Mammalian STE20-like protein kinase 3 antibody
    • Mammalian STE20-like protein kinase 3 C-terminal antibody
    • Mammalian STE20-like protein kinase 3 N-terminal antibody
    • Mammalian sterile 20-like 3 antibody
    • MST-3 antibody
    • MST3/C antibody
    • MST3/N antibody
    • MST3B antibody
    • serine threonine kinase 24 antibody
    • Serine/threonine kinase 24 (Ste20, yeast homolog) antibody
    • Serine/threonine-protein kinase 24 12 kDa subunit antibody
    • Serine/threonine-protein kinase 24 antibody
    • STE20 homolog yeast antibody
    • STE20 like kinase MST3 antibody
    • STE20-like kinase MST3 antibody
    • Stk24 antibody
    • STK24_HUMAN antibody
    • STK3 antibody
    see all

Images

  • Western blot - Anti-MST3 antibody [EP1468Y] (ab51137)
    Western blot - Anti-MST3 antibody [EP1468Y] (ab51137)
    Anti-MST3 antibody [EP1468Y] (ab51137) at 1/5000 dilution + HeLa cell lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP labeled at 1/2000 dilution

    Predicted band size: 49 kDa
    Observed band size: 52 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MST3 antibody [EP1468Y] (ab51137)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MST3 antibody [EP1468Y] (ab51137)

    Ab51137 (1:100) staining human MST3 in human brain tissue by immunohistochemistry using paraffin embedded tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-MST3 antibody [EP1468Y] (ab51137)
    Immunocytochemistry/ Immunofluorescence - Anti-MST3 antibody [EP1468Y] (ab51137)
    Immunofluorescent staining of HeLa cells using ab51137 (1:100).
  • Flow Cytometry - Anti-MST3 antibody [EP1468Y] (ab51137)
    Flow Cytometry - Anti-MST3 antibody [EP1468Y] (ab51137)
    Overlay histogram showing HeLa cells stained with ab51137 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51137, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

References

This product has been referenced in:

  • Tang J  et al. Cdk5-dependent Mst3 phosphorylation and activity regulate neuronal migration through RhoA inhibition. J Neurosci 34:7425-36 (2014). Read more (PubMed: 24872548) »
  • Costa B  et al. STK25 Protein Mediates TrkA and CCM2 Protein-dependent Death in Pediatric Tumor Cells of Neural Origin. J Biol Chem 287:29285-9 (2012). WB ; Human . Read more (PubMed: 22782892) »
See all 4 Publications for this product

Publishing research using ab51137? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Western blot abreview for Anti-MST3 antibody [EP1468Y]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Left ventricle)
Loading amount
50 µg
Specification
Left ventricle
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

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Verified customer

Submitted Jul 28 2009

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