Recombinant
RabMAb

Recombinant Anti-MST3 antibody [EP1468Y] (ab51137)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1468Y] to MST3
  • Suitable for: Flow Cyt, WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MST3 antibody [EP1468Y]
    See all MST3 primary antibodies

  • Description

    Rabbit monoclonal [EP1468Y] to MST3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human MST3 (C terminal). The exact sequence is proprietary.
    (Peptide available as ab198164)

  • Positive control

    • HeLa cells, human brain tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab51137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/5000. Detects a band of approximately 52 kDa (predicted molecular weight: 49 kDa).Can be blocked with Human MST3 peptide (ab198164).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.

Target

  • Function

    Serine/threonine-protein kinase that acts on both serine and threonine residues and promotes apoptosis in response to stress stimuli and caspase activation. Mediates oxidative-stress-induced cell death by modulating phosphorylation of JNK1-JNK2 (MAPK8 and MAPK9), p38 (MAPK11, MAPK12, MAPK13 and MAPK14) during oxidative stress. Plays a role in a staurosporine-induced caspase-independent apoptotic pathway by regulating the nuclear translocation of AIFM1 and ENDOG and the DNase activity associated with ENDOG. Phosphorylates STK38L on 'Thr-442' and stimulates its kinase activity. Regulates cellular migration with alteration of PTPN12 activity and PXN phosphorylation: phosphorylates PTPN12 and inhibits its activity and may regulate PXN phosphorylation through PTPN12. May act as a key regulator of axon regeneration in the optic nerve and radial nerve.

  • Tissue specificity

    Isoform A is ubiquitous. Isoform B is expressed in brain with high expression in hippocampus and cerebral cortex.

  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 protein kinase domain.

  • Post-translational
    modifications

    Proteolytically processed by caspases during apoptosis. Proteolytic cleavage results in kinase activation, nuclear translocation of the truncated form (MST3/N) and the induction of apoptosis.
    Isoform B is activated by phosphorylation by PKA. Oxidative stress induces phosphorylation. Activated by autophosphorylation at Thr-190 and phosphorylation at this site is essential for its function. Manganese, magnesium and cobalt-dependent autophosphorylation is mainly on threonine residues while zinc-dependent autophosphorylation is on both serine and threonine residues.

  • Cellular localization

    Cytoplasm. Nucleus. Membrane. The truncated form (MST3/N) translocates to the nucleus. Co-localizes with STK38L in the membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • epididymis secretory protein Li 95 antibody
    • HEL-S-95 antibody
    • Mammalian STE20 like protein kinase 3 antibody
    • Mammalian STE20-like protein kinase 3 antibody
    • Mammalian STE20-like protein kinase 3 C-terminal antibody
    • Mammalian STE20-like protein kinase 3 N-terminal antibody
    • Mammalian sterile 20-like 3 antibody
    • MST-3 antibody
    • MST3/C antibody
    • MST3/N antibody
    • MST3B antibody
    • serine threonine kinase 24 antibody
    • Serine/threonine kinase 24 (Ste20, yeast homolog) antibody
    • Serine/threonine-protein kinase 24 12 kDa subunit antibody
    • Serine/threonine-protein kinase 24 antibody
    • STE20 homolog yeast antibody
    • STE20 like kinase MST3 antibody
    • STE20-like kinase MST3 antibody
    • Stk24 antibody
    • STK24_HUMAN antibody
    • STK3 antibody
    see all

Images

  • Western blot - Anti-MST3 antibody [EP1468Y] (ab51137)
    Western blot - Anti-MST3 antibody [EP1468Y] (ab51137)
    Anti-MST3 antibody [EP1468Y] (ab51137) at 1/5000 dilution + HeLa cell lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP labeled at 1/2000 dilution

    Predicted band size: 49 kDa
    Observed band size: 52 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MST3 antibody [EP1468Y] (ab51137)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MST3 antibody [EP1468Y] (ab51137)

    Ab51137 (1:100) staining human MST3 in human brain tissue by immunohistochemistry using paraffin embedded tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-MST3 antibody [EP1468Y] (ab51137)
    Immunocytochemistry/ Immunofluorescence - Anti-MST3 antibody [EP1468Y] (ab51137)
    Immunofluorescent staining of HeLa cells using ab51137 (1:100).
  • Flow Cytometry - Anti-MST3 antibody [EP1468Y] (ab51137)
    Flow Cytometry - Anti-MST3 antibody [EP1468Y] (ab51137)
    Overlay histogram showing HeLa cells stained with ab51137 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51137, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (4)

Publishing research using ab51137? Please let us know so that we can cite the reference in this datasheet.

ab51137 has been referenced in 4 publications.

  • Tang J  et al. Cdk5-dependent Mst3 phosphorylation and activity regulate neuronal migration through RhoA inhibition. J Neurosci 34:7425-36 (2014). PubMed: 24872548
  • Costa B  et al. STK25 Protein Mediates TrkA and CCM2 Protein-dependent Death in Pediatric Tumor Cells of Neural Origin. J Biol Chem 287:29285-9 (2012). WB ; Human . PubMed: 22782892
  • Gordon J  et al. Protein phosphatase 2a (PP2A) binds within the oligomerization domain of striatin and regulates the phosphorylation and activation of the mammalian Ste20-Like kinase Mst3. BMC Biochem 12:54 (2011). WB . PubMed: 21985334
  • Zheng X  et al. CCM3 signaling through sterile 20-like kinases plays an essential role during zebrafish cardiovascular development and cerebral cavernous malformations. J Clin Invest 120:2795-804 (2010). WB ; Zebrafish . PubMed: 20592472

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Western blot abreview for Anti-MST3 antibody [EP1468Y]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Left ventricle)
Loading amount
50 µg
Specification
Left ventricle
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Jul 28 2009

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