For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    mst3-antibody-ep1468y-ab51137.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Neuroscience Neurotransmission Intracellular Signaling Kinases
Share by email
Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-MST3 antibody [EP1468Y] (ab51137)

  • Datasheet
Reviews (1) Submit a question References (6)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-MST3 antibody [EP1468Y] (ab51137)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MST3 antibody [EP1468Y] (ab51137)
  • Western blot - Anti-MST3 antibody [EP1468Y] (ab51137)
  • Immunocytochemistry/ Immunofluorescence - Anti-MST3 antibody [EP1468Y] (ab51137)
  • Flow Cytometry (Intracellular) - Anti-MST3 antibody [EP1468Y] (ab51137)
  • Anti-MST3 antibody [EP1468Y] (ab51137)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1468Y] to MST3
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Kit
Product image
Rap1 Activation Assay Kit (ab212011)
Knockout
Product image
Human STK24 knockout A-431 cell line (ab269479)

View more associated products

Overview

  • Product name

    Anti-MST3 antibody [EP1468Y]
    See all MST3 primary antibodies
  • Description

    Rabbit monoclonal [EP1468Y] to MST3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab198164)

  • Positive control

    • A431, HeLa and Raji cell lysates; HeLa cells; Human brain tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1468Y
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Intracellular Signaling
    • Kinases
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-MST3 antibody [EP1468Y] - BSA and Azide free (ab247312)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Immunizing Peptide (Blocking)

    • Human MST3 peptide (ab198164)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human STK24 knockout A-431 cell line (ab269479)
  • KO cell lysates

    • Human STK24 knockout A-431 cell lysate (ab269643)
  • Recombinant Protein

    • Recombinant human MST3 protein (ab51413)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab51137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB (1)
1/5000. Detects a band of approximately 52 kDa (predicted molecular weight: 49 kDa).Can be blocked with Human MST3 peptide (ab198164).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
Notes
Flow Cyt (Intra)
1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/5000. Detects a band of approximately 52 kDa (predicted molecular weight: 49 kDa).Can be blocked with Human MST3 peptide (ab198164).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.

Target

  • Function

    Serine/threonine-protein kinase that acts on both serine and threonine residues and promotes apoptosis in response to stress stimuli and caspase activation. Mediates oxidative-stress-induced cell death by modulating phosphorylation of JNK1-JNK2 (MAPK8 and MAPK9), p38 (MAPK11, MAPK12, MAPK13 and MAPK14) during oxidative stress. Plays a role in a staurosporine-induced caspase-independent apoptotic pathway by regulating the nuclear translocation of AIFM1 and ENDOG and the DNase activity associated with ENDOG. Phosphorylates STK38L on 'Thr-442' and stimulates its kinase activity. Regulates cellular migration with alteration of PTPN12 activity and PXN phosphorylation: phosphorylates PTPN12 and inhibits its activity and may regulate PXN phosphorylation through PTPN12. May act as a key regulator of axon regeneration in the optic nerve and radial nerve.
  • Tissue specificity

    Isoform A is ubiquitous. Isoform B is expressed in brain with high expression in hippocampus and cerebral cortex.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Proteolytically processed by caspases during apoptosis. Proteolytic cleavage results in kinase activation, nuclear translocation of the truncated form (MST3/N) and the induction of apoptosis.
    Isoform B is activated by phosphorylation by PKA. Oxidative stress induces phosphorylation. Activated by autophosphorylation at Thr-190 and phosphorylation at this site is essential for its function. Manganese, magnesium and cobalt-dependent autophosphorylation is mainly on threonine residues while zinc-dependent autophosphorylation is on both serine and threonine residues.
  • Cellular localization

    Cytoplasm. Nucleus. Membrane. The truncated form (MST3/N) translocates to the nucleus. Co-localizes with STK38L in the membrane.
  • Target information above from: UniProt accession Q9Y6E0 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8428 Human
    • Entrez Gene: 223255 Mouse
    • Entrez Gene: 361092 Rat
    • Omim: 604984 Human
    • SwissProt: Q9Y6E0 Human
    • SwissProt: Q99KH8 Mouse
    • SwissProt: B0LT89 Rat
    • Unigene: 508514 Human
    • Unigene: 390756 Mouse
    • Unigene: 442081 Mouse
    • Unigene: 472866 Mouse
    • Unigene: 105397 Rat
    see all
  • Alternative names

    • epididymis secretory protein Li 95 antibody
    • HEL-S-95 antibody
    • Mammalian STE20 like protein kinase 3 antibody
    • Mammalian STE20-like protein kinase 3 antibody
    • Mammalian STE20-like protein kinase 3 C-terminal antibody
    • Mammalian STE20-like protein kinase 3 N-terminal antibody
    • Mammalian sterile 20-like 3 antibody
    • MST-3 antibody
    • MST3/C antibody
    • MST3/N antibody
    • MST3B antibody
    • serine threonine kinase 24 antibody
    • Serine/threonine kinase 24 (Ste20, yeast homolog) antibody
    • Serine/threonine-protein kinase 24 12 kDa subunit antibody
    • Serine/threonine-protein kinase 24 antibody
    • STE20 homolog yeast antibody
    • STE20 like kinase MST3 antibody
    • STE20-like kinase MST3 antibody
    • Stk24 antibody
    • STK24_HUMAN antibody
    • STK3 antibody
    see all

Images

  • Western blot - Anti-MST3 antibody [EP1468Y] (ab51137)
    Western blot - Anti-MST3 antibody [EP1468Y] (ab51137)
    All lanes : Anti-MST3 antibody [EP1468Y] (ab51137) at 1/1000 dilution

    Lane 1 : Wild-type A431 cell lysate
    Lane 2 : STK24 knockout A431 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Raji cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 49 kDa
    Observed band size: 52 kDa why is the actual band size different from the predicted?



    Lanes 1 - 4: Merged signal (red and green). Green - ab51137 observed at 52 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

    ab51137 was shown to react with MST3 in wild-type A431 cells in Western blot with loss of signal observed in STK24 knockout cell line ab269479 (knockout cell lysate ab269643). Wild-type A431 and STK24 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab51137 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MST3 antibody [EP1468Y] (ab51137)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MST3 antibody [EP1468Y] (ab51137)

    Ab51137 (1:100) staining human MST3 in human brain tissue by immunohistochemistry using paraffin embedded tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-MST3 antibody [EP1468Y] (ab51137)
    Western blot - Anti-MST3 antibody [EP1468Y] (ab51137)
    Anti-MST3 antibody [EP1468Y] (ab51137) at 1/5000 dilution + HeLa cell lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP labeled at 1/2000 dilution

    Predicted band size: 49 kDa
    Observed band size: 52 kDa why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-MST3 antibody [EP1468Y] (ab51137)
    Immunocytochemistry/ Immunofluorescence - Anti-MST3 antibody [EP1468Y] (ab51137)
    Immunofluorescent staining of HeLa cells using ab51137 (1:100).
  • Flow Cytometry (Intracellular) - Anti-MST3 antibody [EP1468Y] (ab51137)
    Flow Cytometry (Intracellular) - Anti-MST3 antibody [EP1468Y] (ab51137)

    Overlay histogram showing HeLa cells stained with ab51137 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51137, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

     

  • Anti-MST3 antibody [EP1468Y] (ab51137)
    Anti-MST3 antibody [EP1468Y] (ab51137)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (6)

Publishing research using ab51137? Please let us know so that we can cite the reference in this datasheet.

ab51137 has been referenced in 6 publications.

  • Yang J  et al. STK24 modulates excitatory synaptic transmission in epileptic hippocampal neurons. CNS Neurosci Ther 26:851-861 (2020). PubMed: 32436359
  • Zalvide J  et al. Measuring the Kinase Activity of GCKIII Proteins In Vitro. Methods Mol Biol 2152:437-443 (2020). PubMed: 32524571
  • Tang J  et al. Cdk5-dependent Mst3 phosphorylation and activity regulate neuronal migration through RhoA inhibition. J Neurosci 34:7425-36 (2014). PubMed: 24872548
  • Costa B  et al. STK25 Protein Mediates TrkA and CCM2 Protein-dependent Death in Pediatric Tumor Cells of Neural Origin. J Biol Chem 287:29285-9 (2012). WB ; Human . PubMed: 22782892
  • Gordon J  et al. Protein phosphatase 2a (PP2A) binds within the oligomerization domain of striatin and regulates the phosphorylation and activation of the mammalian Ste20-Like kinase Mst3. BMC Biochem 12:54 (2011). WB . PubMed: 21985334
  • Zheng X  et al. CCM3 signaling through sterile 20-like kinases plays an essential role during zebrafish cardiovascular development and cerebral cavernous malformations. J Clin Invest 120:2795-804 (2010). WB ; Zebrafish . PubMed: 20592472

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Western blot abreview for Anti-MST3 antibody [EP1468Y]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Left ventricle)
Loading amount
50 µg
Specification
Left ventricle
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Jul 28 2009

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2022 Abcam plc. All rights reserved.