Product nameAnti-MST4 antibody [EPR12766]
See all MST4 primary antibodies
DescriptionRabbit monoclonal [EPR12766] to MST4
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: ICC or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human MST4 aa 350 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q9P289
- HeLa, 293T, Jurkat, Human fetal brain, PC12 and NIH 3T3 lysates. Human gastric carcinoma and prostate tissues. Permeabilized Jurkat cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab177153 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 52 kDa (predicted molecular weight: 47 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionMediator of cell growth. Modulates apoptosis.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 protein kinase domain.
Cellular localizationCytoplasm. Golgi apparatus membrane. Localizes to the Golgi apparatus.
- Information by UniProt
- 2610018G03Rik antibody
- EC 22.214.171.124 antibody
- Mammalian Ste20 like protein kinase 4 antibody
All lanes : Anti-MST4 antibody [EPR12766] (ab177153) at 1/1000 dilution
Lane 1 : HeLa lysate
Lane 2 : 293T lysate
Lane 3 : Jurkat lysate
Lane 4 : Human fetal brain lysate
Lane 5 : PC12 lysate
Lane 6 : NIH 3T3 lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 47 kDa
Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling MST4 with ab177153 at 1/50 dilution.
Flow cytometric analysis of permeabilized Jurkat cells labeling MST4 with ab177153 at 1/10 dilution (red), or a rabbit IgG (negative) (green).
Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling MST4 with ab177153 at 1/50 dilution.
ab177153 has not yet been referenced specifically in any publications.