Recombinant Anti-MST4 antibody [EPR12766] (ab177153)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12766] to MST4
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-MST4 antibody [EPR12766]
See all MST4 primary antibodies -
Description
Rabbit monoclonal [EPR12766] to MST4 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, 293T, Jurkat, Human fetal brain, PC12 and NIH 3T3 lysates. Human gastric carcinoma and prostate tissues. Permeabilized Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12766 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab177153 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/5000. Detects a band of approximately 52 kDa (predicted molecular weight: 47 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/5000. Detects a band of approximately 52 kDa (predicted molecular weight: 47 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Mediator of cell growth. Modulates apoptosis. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 protein kinase domain. -
Cellular localization
Cytoplasm. Golgi apparatus membrane. Localizes to the Golgi apparatus. - Information by UniProt
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Database links
- Entrez Gene: 51765 Human
- Entrez Gene: 70415 Mouse
- Entrez Gene: 317589 Rat
- Omim: 300547 Human
- SwissProt: Q9P289 Human
- SwissProt: Q99JT2 Mouse
- Unigene: 444247 Human
- Unigene: 391246 Mouse
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Alternative names
- 2610018G03Rik antibody
- EC 2.7.11.1 antibody
- Mammalian Ste20 like protein kinase 4 antibody
see all
Images
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All lanes : Anti-MST4 antibody [EPR12766] (ab177153) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Human fetal brain tissue lysate
Lane 5 : PC-12 cell lysate
Lane 6 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 47 kDa -
Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling MST4 with ab177153 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Intracellular flow cytometric analysis of permeabilized Jurkat cellslabeling MST4with ab177153 at 1/10 dilution (red), or a rabbit IgG (negative) (green).
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Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling MST4 with ab177153 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab177153 has not yet been referenced specifically in any publications.