Anti-MST4 + MST3 + STK25 (phospho T174 + T178 + T190) antibody [EP2123Y] (ab76579)


  • Product name
    Anti-MST4 + MST3 + STK25 (phospho T174 + T178 + T190) antibody [EP2123Y]
  • Description
    Rabbit monoclonal [EP2123Y] to MST4 + MST3 + STK25 (phospho T174 + T178 + T190)
  • Host species
  • Tested applications
    Suitable for: WB, ICCmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding threonine 178 of human MST4, theonine 190 of human MST3 and threonine 174 of human STK25.

  • Positive control
    • HeLa cell lysates treated with calyculin A.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
  • Clone number
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab76579 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    ICC: 1/100 - 1/250.
    WB: 1/1000 - 1/2000. Detects a band of approximately 52 kDa (predicted molecular weight: MST3, 49 kDa; MST4, 47 kDa & STK25, 48 kDa).

    Is unsuitable for Flow Cyt, IHC-P or IP.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Novel human Ste20-related kinase Mst4 is biologically active in the activation of MEK/ERK pathway and in mediating cell growth and transformation. It is pro apoptotic and is highly expressed in placenta, thymus, and peripheral blood leukocytes. Interaction with Golgi matrix protein GOLGA2 results in autophosphorylation on Thr-178, possibly as a consequence of stabilization of dimer formation. This may also be activated by C terminal cleavage. MST3 or Mammalian Sterile 20-like kinase 3 is a member of the germinal center kinase-III family. MST3 contains a conserved kinase domain at its NH(2)-terminus and a regulatory domain at its COOH-terminus. Caspase-mediated cleavage of the regulatory domain of MST3 activates its intrinsic kinase activity and leads to nuclear translocation. Expression of COOH-terminal truncated MST3 in cells results in DNA fragmentation and induction of apoptosis. It can inhibit cell migration in a fashion dependent on autophosphorylation and can regulate paxillin phosphorylation through tyrosine phosphatase PTP-PEST. Mitogen activated protein kinase cascades have been conserved throughout evolution. In mammals, these cascades allow responses to complex stimuli such as growth factors and inflammatory cytokines. In yeast, STK25 functions upstream of the MAPK cascade.
    • Cellular localization
      STK25 and MST4: Cytoplasmic, Golgi Apparatus MST3: Cytoplasmic
    • Database links
    • Alternative names
      • 0610042I06Rik antibody
      • 1500019J11Rik antibody
      • AU018434 antibody
      • C86992 antibody
      • DKFZp686J1430 antibody
      • Mammalian STE20 like protein kinase 3 antibody
      • Mammalian Ste20 like protein kinase 4 antibody
      • Mammalian sterile 20 like 4 antibody
      • MASK antibody
      • mess1 antibody
      • MGC94619 antibody
      • MST 3 antibody
      • MST 4 antibody
      • MST antibody
      • Mst2 antibody
      • MST3 and SOK1 related kinase antibody
      • MST3 antibody
      • MST3B antibody
      • OTTHUMP00000018592 antibody
      • OTTHUMP00000018593 antibody
      • Protein kinase homolog serine/threonine kinase 3 (Ste20 yeast homolog) antibody
      • RGD1563568 antibody
      • RP11-111L24.5 antibody
      • Serine/threonine kinase 24 (STE20 homolog yeast) antibody
      • Serine/threonine kinase 24 antibody
      • Serine/threonine kinase 25 antibody
      • Serine/threonine protein kinase 24 antibody
      • Serine/threonine protein kinase MASK antibody
      • Serine/threonine protein kinase MST 4 antibody
      • Serine/threonine protein kinase MST4 antibody
      • SOK1 antibody
      • STE20 antibody
      • Ste20 like antibody
      • Ste20 like kinase antibody
      • STE20 like kinase 3 antibody
      • STE20 like kinase MST 4 antibody
      • STE20 like kinase MST3 antibody
      • STE20 like kinase MST4 antibody
      • Ste20 yeast homolog antibody
      • Ste20/oxidant stress response kinase 1 antibody
      • Sterile 20 (oxidant stress response kinase 1) antibody
      • sterile 20 like kinase 3 antibody
      • Sterile 20/oxidant stress-response kinase 1 antibody
      • STK3 antibody
      • Yeast Sps1/Ste20 related kinase 1 antibody
      • YSK1 antibody
      see all


    • All lanes : Anti-MST4 + MST3 + STK25 (phospho T174 + T178 + T190) antibody [EP2123Y] (ab76579) at 1/2000 dilution

      Lane 1 : HeLa cell lysates, untreated
      Lane 2 : HeLa cell lysates, treated with calyculin A

      Lysates/proteins at 10 µg per lane.

      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 47-49 kDa
      Observed band size: 52 kDa (why is the actual band size different from the predicted?)


    This product has been referenced in:
    • Mardakheh FK  et al. RHO binding to FAM65A regulates Golgi reorientation during cell migration. J Cell Sci 129:4466-4479 (2016). WB ; Human . Read more (PubMed: 27807006) »
    • Costa B  et al. STK25 Protein Mediates TrkA and CCM2 Protein-dependent Death in Pediatric Tumor Cells of Neural Origin. J Biol Chem 287:29285-9 (2012). WB ; Human . Read more (PubMed: 22782892) »

    See all 4 Publications for this product

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