Overview

  • Product name
  • Description
    Rabbit polyclonal to MSX1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide within Human MSX1 aa 111-138 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P28360

  • Positive control

Properties

Applications

Our Abpromise guarantee covers the use of ab174207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 31 kDa.
ICC/IF 1/200.

Target

  • Function
    Acts as a transcriptional repressor. May play a role in limb-pattern formation. Acts in cranofacial development and specifically in odontogenesis. Expression in the developing nail bed mesenchyme is important for nail plate thickness and integrity.
  • Tissue specificity
    Expressed in the developing nail bed mesenchyme.
  • Involvement in disease
    Defects in MSX1 are the cause of tooth agenesis selective type 1 (STHAG1) [MIM:106600]. A form of selective tooth agenesis, a common anomaly characterized by the congenital absence of one or more teeth. Selective tooth agenesis without associated systemic disorders has sometimes been divided into 2 types: oligodontia, defined as agenesis of 6 or more permanent teeth, and hypodontia, defined as agenesis of less than 6 teeth. The number in both cases does not include absence of third molars (wisdom teeth). Tooth agenesis selective type 1 can be associated with orofacial cleft in some patients.
    Note=MSX1 is deleted in some patients with Wolf-Hirschhorn syndrome (WHS). WHS results from sub-telomeric deletions in the short arm of chromosome 4.
    Defects in MSX1 are the cause of Witkop syndrome (WITS) [MIM:189500]. WITS is a form of ectodermal dyslasia also called tooth-and-nail syndrome or dysplasia of nails with hypodontia. Ectodermal dysplasias (EDs) constitute a heterogeneous group of developmental disorders affecting tissues of ectodermal origin. EDs are characterized by abnormal development of two or more ectodermal structures such as hair, teeth, nails and sweat glands, with or without any additional clinical sign. Each combination of clinical features represents a different type of ectodermal dysplasia. Witkop syndrome is characterized by abnormalities largely limited largely to teeth (some of which are missing) and nails (which are poorly formed early in life, especially toenails). This condition is distinguished from anhidrotic ectodermal dysplasia by autosomal dominant inheritance and little involvement of hair and sweat glands. The teeth are not as severely affected.
    Defects in MSX1 are the cause of non-syndromic orofacial cleft type 5 (OFC5) [MIM:608874]; also called non-syndromic cleft lip with or without cleft palate 5. Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum.
  • Sequence similarities
    Belongs to the Msh homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Post-translational
    modifications
    Sumoylated by PIAS1, desumoylated by SENP1.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AA675338 antibody
    • AI324650 antibody
    • Homeobox 7 antibody
    • Homeobox protein Hox-7 antibody
    • Homeobox protein MSX 1 antibody
    • Homeobox protein MSX-1 antibody
    • Homeobox protein MSX1 antibody
    • Homeobox, msh like 1 antibody
    • Homeobox, msh-like 1 antibody
    • HOX 7 antibody
    • Hox 7.1 antibody
    • Hox-7 antibody
    • HOX7 antibody
    • Hox7.1 antibody
    • HYD 1 antibody
    • HYD1 antibody
    • msh (Drosophila) homeo box homolog 1 (formerly homeo box 7) antibody
    • Msh antibody
    • msh homeo box 1 antibody
    • msh homeo box homolog 1 antibody
    • Msh homeobox 1 antibody
    • Msh homeobox 1 like protein antibody
    • Msh homeobox 1-like protein antibody
    • msh homeobox homolog 1 (Drosophila) antibody
    • msh homeobox homolog 1 antibody
    • MSH, Drosophila, Homolog of, 1 antibody
    • MSX 1 antibody
    • MSX1 antibody
    • MSX1_HUMAN antibody
    • Muscle segment homeobox antibody
    • Muscle segment homeobox, Drosophila, Homolog of, 1 antibody
    • OFC5 antibody
    • OTTHUMP00000115387 antibody
    • STHAG1 antibody
    see all

Images

  • Anti-MSX1 antibody (ab174207) + Mouse spleen tissue lysate at 35 µg

    Predicted band size: 31 kDa

  • Anti-MSX1 antibody (ab174207) + A375 cell lysate at 35 µg

    Predicted band size: 31 kDa

  • Immunocytochemistry/Immunofluorescence analysis of U251 cells labelling MSX1 (green) with ab174207. Cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with the primary antibody (1/25, 1 h at 37°C). An Alexa Fluor® 488 conjugated donkey anti-rabbit IgG was used as the secondary antibody (1/400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). MSX1 immunoreactivity is localized to the nucleus significantly.

  • Immunocytochemistry/Immunofluorescence analysis of SY5Y cells (4% PFA fixed, permeabilized with Triton X-100) labeling MSX1 (green) with ab174207 at 1/200 dilution. An Alexa Fluor® 488 conjugated donkey anti-rabbit IgG was used as the secondary antibody. Nuclei were counterstained with Hoechst 33342 (blue).

References

ab174207 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (differentiated human ES cells)
Permeabilization
Yes - PBS/0.1% Triton X-100
Specification
differentiated human ES cells
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Formaldehyde
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Submitted Dec 23 2016

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