Overview

  • Product name

  • Description

    Rabbit polyclonal to Msx2/Hox8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Cow, Chimpanzee, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Msx2/Hox8 (internal sequence). The exact sequence is proprietary.
    Database link: P35548

  • Positive control

    • WB: HEK-293T, A431, HeLa, HepG2, Neuro-2a, C8D30, NIH/3T3, RAW 264.7, C2C12 and HL-60 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab227720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 29 kDa.

Target

  • Function

    Acts as a transcriptional regulator in bone development. Represses the ALPL promoter activity and antogonizes the stimulatory effect of DLX5 on ALPL expression during osteoblast differentiation. Probable morphogenetic role. May play a role in limb-pattern formation. In osteoblasts, suppresses transcription driven by the osteocalcin FGF response element (OCFRE). Binds to the homeodomain-response element of the ALPL promoter.
  • Involvement in disease

    Defects in MSX2 are the cause of parietal foramina 1 (PFM1) [MIM:168500]; also known as foramina parietalia permagna (FPP). PFM1 is an autosomal dominant disease characterized by oval defects of the parietal bones caused by deficient ossification around the parietal notch, which is normally obliterated during the fifth fetal month.
    Defects in MSX2 are the cause of parietal foramina with cleidocranial dysplasia (PFMCCD) [MIM:168550]; also known as cleidocranial dysplasia with parietal foramina. PFMCCD combines skull defects in the form of enlarged parietal foramina and deficient ossification of the clavicles.
    Defects in MSX2 are the cause of craniosynostosis type 2 (CRS2) [MIM:604757]; also known as craniosynostosis Boston-type (CSB). CRS2 is an autosomal dominant disorder characterized by the premature fusion of calvarial sutures. The craniosynostosis phenotype is either fronto-orbital recession, or frontal bossing, or turribrachycephaly, or cloverleaf skull. Associated features include severe headache, high incidence of visual problems (myopia or hyperopia), and short first metatarsals. Intelligence is normal.
  • Sequence similarities

    Belongs to the Msh homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CRS 2 antibody
    • CRS2 antibody
    • FPP antibody
    • Homeo box msh like 2 antibody
    • Homeobox protein Hox-8 antibody
    • Homeobox protein MSX 2 antibody
    • Homeobox protein MSX-2 antibody
    • Homeobox protein MSX2 antibody
    • Hox 8 antibody
    • Hox8 antibody
    • MSH antibody
    • Msh homeo box 2 antibody
    • Msh homeo box homolog antibody
    • Msh homeo box homolog 2 antibody
    • Msh homeobox 2 antibody
    • Msh homeobox homolog 2 antibody
    • Msx 2 antibody
    • MSX2 antibody
    • MSX2_HUMAN antibody
    • Parietal foramina 1 antibody
    • PFM 1 antibody
    • PFM antibody
    • PFM1 antibody
    see all

Images

  • All lanes : Anti-Msx2/Hox8 antibody (ab227720) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 29 kDa



    12% SDS-PAGE

  • All lanes : Anti-Msx2/Hox8 antibody (ab227720) at 1/1000 dilution

    Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate
    Lane 2 : C8D30 whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
    Lane 4 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 5 : C2C12 (mouse myoblast cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 29 kDa



    12% SDS-PAGE

  • Anti-Msx2/Hox8 antibody (ab227720) at 1/500 dilution + HL-60 (human promyelocytic leukemia cell line) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 29 kDa



    12% SDS-PAGE

References

ab227720 has not yet been referenced specifically in any publications.

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