Recombinant
RabMAb

Recombinant Anti-MSY2/YBOX2/YBX2 antibody [EPR2814(2)] (ab154829)

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Overview

  • Product name

    Anti-MSY2/YBOX2/YBX2 antibody [EPR2814(2)]
    See all MSY2/YBOX2/YBX2 primary antibodies

  • Description

    Rabbit monoclonal [EPR2814(2)] to MSY2/YBOX2/YBX2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to Human MSY2/YBOX2/YBX2.
    Database link: Q9Y2T7

  • Positive control

    • Human seminoma, Mouse testis and Rat testis lysates; HepG2 cells.

  • General notes

     

     

     This product was previously labelled as MSY2/YBOX2

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab154829 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    Images

    • Western blot - Anti-MSY2/YBOX2/YBX2 antibody [EPR2814(2)] (ab154829)
      Western blot - Anti-MSY2/YBOX2/YBX2 antibody [EPR2814(2)] (ab154829)
      All lanes : Anti-MSY2/YBOX2/YBX2 antibody [EPR2814(2)] (ab154829) at 1/1000 dilution

      Lane 1 : Human seminoma lysate
      Lane 2 : Mouse testis lysate
      Lane 3 : Rat testis lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 38 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-MSY2/YBOX2/YBX2 antibody [EPR2814(2)] (ab154829)
      Immunocytochemistry/ Immunofluorescence - Anti-MSY2/YBOX2/YBX2 antibody [EPR2814(2)] (ab154829)

      Immunofluorescent analysis of HepG2 cells labeling MSY2/YBOX2/YBX2 with ab154829 at 1/50 dilution.

    References

    This product has been referenced in:

    • He Y  et al. Murine PAIP1 stimulates translation of spermiogenic mRNAs stored by YBX2 via its interaction with YBX2†. Biol Reprod 100:561-572 (2019). Read more (PubMed: 30295753) »
    See 1 Publication for this product

    Publishing research using ab154829? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MSY2/YBOX2 antibody [EPR2814(2)]

     Poor
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Marmoset (common) Tissue sections (Adult ovary)
    Specification
    Adult ovary
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval solution
    Permeabilization
    No
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Dr. 煜欽 林

    Verified customer

    Submitted May 13 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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