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RecombinantRabMAb

Recombinant Anti-MSY2/YBOX2/YBX2 antibody [EPR2814(2)] (ab154829)

  • Datasheet
Reviews (1) Submit a question References (2)

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Find out more.

Western blot - Anti-MSY2/YBOX2/YBX2 antibody [EPR2814(2)] (ab154829)
  • Immunocytochemistry/ Immunofluorescence - Anti-MSY2/YBOX2/YBX2 antibody [EPR2814(2)] (ab154829)
  • Anti-MSY2/YBOX2/YBX2 antibody [EPR2814(2)] (ab154829)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2814(2)] to MSY2/YBOX2/YBX2
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-MSY2/YBOX2/YBX2 antibody [EPR2814(2)]
    See all MSY2/YBOX2/YBX2 primary antibodies
  • Description

    Rabbit monoclonal [EPR2814(2)] to MSY2/YBOX2/YBX2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human MSY2/YBOX2/YBX2.
    Database link: Q9Y2T7

  • Positive control

    • Human seminoma, Mouse testis and Rat testis lysates; HepG2 cells.
  • General notes

     

     

     This product was previously labelled as MSY2/YBOX2

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR2814(2)
  • Isotype

    IgG
  • Research areas

    • Stem Cells
    • Germline Stem Cells
    • Embryonic Germ Cells
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding
    • Developmental Biology
    • Reproduction
    • Germ cell markers

Associated products

  • Alternative Versions

    • Anti-MSY2/YBOX2/YBX2 antibody [EPR2814(2)] - BSA and Azide free (ab249135)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Mouse testis normal tissue lysate - total protein (ab29289)
  • Recombinant Protein

    • Recombinant Human MSY2/YBOX2/YBX2 protein (ab162411)

Applications

Our Abpromise guarantee covers the use of ab154829 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Target information above from: UniProt accession Q9Y2T7 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 51087 Human
      • Entrez Gene: 303250 Rat
      • Omim: 611447 Human
      • SwissProt: Q9Y2T7 Human
      • SwissProt: Q9Z2C8 Mouse
      • Unigene: 567494 Human
      • Unigene: 29286 Mouse
      • Alternative names

        • Contrin antibody
        • CSDA 3 antibody
        • CSDA3 antibody
        • Dbpc antibody
        • DNA binding protein C antibody
        • DNA-binding protein C antibody
        • FRGY2 homolog antibody
        • Germ cell specific Y box binding protein antibody
        • Germ cell-specific Y-box-binding protein antibody
        • MGC118270 antibody
        • MGC45104 antibody
        • MSY 2 antibody
        • MSY2 antibody
        • MSY2 homolog antibody
        • OTTMUSP00000006276 antibody
        • RGD1305068 antibody
        • Y box binding protein 2 antibody
        • Y-box-binding protein 2 antibody
        • YBOX2_HUMAN antibody
        • YBX 2 antibody
        • YBX2 antibody
        see all

      Images

      • Western blot - Anti-MSY2/YBOX2/YBX2 antibody [EPR2814(2)] (ab154829)
        Western blot - Anti-MSY2/YBOX2/YBX2 antibody [EPR2814(2)] (ab154829)
        All lanes : Anti-MSY2/YBOX2/YBX2 antibody [EPR2814(2)] (ab154829) at 1/1000 dilution

        Lane 1 : Human seminoma lysate
        Lane 2 : Mouse testis lysate
        Lane 3 : Rat testis lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 38 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-MSY2/YBOX2/YBX2 antibody [EPR2814(2)] (ab154829)
        Immunocytochemistry/ Immunofluorescence - Anti-MSY2/YBOX2/YBX2 antibody [EPR2814(2)] (ab154829)

        Immunofluorescent analysis of HepG2 cells labeling MSY2/YBOX2/YBX2 with ab154829 at 1/50 dilution.

      • Anti-MSY2/YBOX2/YBX2 antibody [EPR2814(2)] (ab154829)
        Anti-MSY2/YBOX2/YBX2 antibody [EPR2814(2)] (ab154829)

      Protocols

      • Western blot protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (2)

      Publishing research using ab154829? Please let us know so that we can cite the reference in this datasheet.

      ab154829 has been referenced in 2 publications.

      • He Y  et al. Murine PAIP1 stimulates translation of spermiogenic mRNAs stored by YBX2 via its interaction with YBX2†. Biol Reprod 100:561-572 (2019). PubMed: 30295753
      • Chen F  et al. LINC00958 regulated miR-627-5p/YBX2 axis to facilitate cell proliferation and migration in oral squamous cell carcinoma. Cancer Biol Ther 20:1270-1280 (2019). PubMed: 31161900

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MSY2/YBOX2 antibody [EPR2814(2)]

      Poor
      Abreviews
      Abreviews
      This product is known to not work in this application or species.
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Marmoset (common) Tissue sections (Adult ovary)
      Specification
      Adult ovary
      Fixative
      Formaldehyde
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval solution
      Permeabilization
      No
      Blocking step
      Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Dr. 煜欽 林

      Verified customer

      Submitted May 13 2013

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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