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Signal Transduction Metabolism Mitochondrial
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Anti-MT-CYB antibody [5B3-6E3] - N-terminal (ab219823)

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Western blot - Anti-MT-CYB antibody [5B3-6E3] - N-terminal (ab219823)
  • Western blot - Anti-MT-CYB antibody [5B3-6E3] - N-terminal (ab219823)

Key features and details

  • Mouse monoclonal [5B3-6E3] to MT-CYB - N-terminal
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG2b

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Overview

  • Product name

    Anti-MT-CYB antibody [5B3-6E3] - N-terminal
    See all MT-CYB primary antibodies
  • Description

    Mouse monoclonal [5B3-6E3] to MT-CYB - N-terminal
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human MT-CYB (N terminal).
    Database link: P00156

  • Positive control

    • WB: Mitochondria from cultured normal control human dermal fibroblasts neonatal (HDFn); Whole cell extract of cultured normal control human dermal fibroblasts neonatal (HDFn).
  • General notes

    Western blot advice:

    Hydrophobic intrinsic membrane proteins such as the core mtDNA-encoded proteins of the mitochondrial OXPHOS complexes tend to run faster in SDS-PAGE than predicted by their amino acid composition. This is likely due to incomplete unfolding of the protein and a more negative charge:mass ratio.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 0.36% HEPES, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity

    Protein L purified
  • Purification notes

    Purified from hybridoma cell culture supernatant by Protein L affinity chromatography from fetal bovine serum containing medium (Protein L does not bind bovine IgG).
  • Clonality

    Monoclonal
  • Clone number

    5B3-6E3
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Antioxidants

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab219823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 0.05 - 2 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 43 kDa).
Notes
WB
Use a concentration of 0.05 - 2 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 43 kDa).

Target

  • Function

    Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.
  • Involvement in disease

    Defects in MT-CYB are a rare cause of mitochondrial dysfunction underlying different myopathies. They include mitochondrial encephalomyopathy, hypertrophic cardiomyopathy (HCM), and sporadic mitochondrial myopathy (MM). In mitochondrial myopathy, exercise intolerance is the predominant symptom. Additional features include lactic acidosis, muscle weakness and/or myoglobinuria. Defects in MTCYB are also found in cases of exercise intolerance accompanied by deafness, mental retardation, retinitis pigmentosa, cataract, growth retardation, epilepsy (multisystem disorder).
    Cardiomyopathy, infantile histiocytoid
    Leber hereditary optic neuropathy
  • Sequence similarities

    Belongs to the cytochrome b family.
  • Cellular localization

    Mitochondrion inner membrane.
  • Target information above from: UniProt accession P00156 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4519 Human
    • SwissProt: P00156 Human
    • Alternative names

      • COB antibody
      • Complex III subunit 3 antibody
      • Complex III subunit III antibody
      • CYB_HUMAN antibody
      • CYTB antibody
      • Cytochrome b antibody
      • Cytochrome b c1 complex subunit 3 antibody
      • Cytochrome b-c1 complex subunit 3 antibody
      • Mitochondrially encoded cytochrome b antibody
      • MT-CYB antibody
      • MTCYB antibody
      • Ubiquinol cytochrome c reductase complex cytochrome b subunit antibody
      • Ubiquinol-cytochrome-c reductase complex cytochrome b subunit antibody
      see all

    Images

    • Western blot - Anti-MT-CYB antibody [5B3-6E3] - N-terminal (ab219823)
      Western blot - Anti-MT-CYB antibody [5B3-6E3] - N-terminal (ab219823)
      All lanes : Anti-MT-CYB antibody [5B3-6E3] - N-terminal (ab219823) at 0.1 µg/ml

      Lane 1 : Mitochondria from cultured normal control human dermal fibroblasts neonatal (HDFn)
      Lane 2 : Mitochondria from HDFn cells depleted of mtDNA by long-term proliferation in the presence of ethidium bromide

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-labeled Goat-anti-mouse IgG

      Developed using the ECL technique.

      Predicted band size: 43 kDa
      Observed band size: 28 kDa why is the actual band size different from the predicted?



      Mitochondrial proteins solubilized in 2% SDS were separated by SDS-PAGE and then transferred to PVDF membranes in CAPS buffer.

    • Western blot - Anti-MT-CYB antibody [5B3-6E3] - N-terminal (ab219823)
      Western blot - Anti-MT-CYB antibody [5B3-6E3] - N-terminal (ab219823)
      All lanes : Anti-MT-CYB antibody [5B3-6E3] - N-terminal (ab219823) at 2 µg/ml

      Lane 1 : Whole cell extract of cultured normal control human dermal fibroblasts neonatal (HDFn)
      Lane 2 : Whole cell extract HDFn-Rho0 cells depleted of mtDNA by long-term culture in the presence of ethidium bromide

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : HRP-labeled Goat-anti-mouse IgG

      Developed using the ECL technique.

      Predicted band size: 43 kDa
      Observed band size: 28 kDa why is the actual band size different from the predicted?



      Proteins were separated by SDS-PAGE and then transferred to PVDF membranes in CAPS buffer.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab219823? Please let us know so that we can cite the reference in this datasheet.

    ab219823 has not yet been referenced specifically in any publications.

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