Anti-MT-CYB antibody [5B3-6E3] - N-terminal (ab219823)
![Western blot - Anti-MT-CYB antibody [5B3-6E3] - N-terminal (ab219823)](https://www.abcam.com/ps/products/219/ab219823/Images/ab219823-286096-anti-mt-cyb-antibody-5b3-6e3-n-terminal-western-blot.jpg "Western blot - Anti-MT-CYB antibody [5B3-6E3] - N-terminal (ab219823)")
Key features and details
- Mouse monoclonal [5B3-6E3] to MT-CYB - N-terminal
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-MT-CYB antibody [5B3-6E3] - N-terminal
See all MT-CYB primary antibodies -
Description
Mouse monoclonal [5B3-6E3] to MT-CYB - N-terminal -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human MT-CYB (N terminal).
Database link: P00156 -
Positive control
- WB: Mitochondria from cultured normal control human dermal fibroblasts neonatal (HDFn); Whole cell extract of cultured normal control human dermal fibroblasts neonatal (HDFn).
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General notes
Western blot advice:
Hydrophobic intrinsic membrane proteins such as the core mtDNA-encoded proteins of the mitochondrial OXPHOS complexes tend to run faster in SDS-PAGE than predicted by their amino acid composition. This is likely due to incomplete unfolding of the protein and a more negative charge:mass ratio.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 0.36% HEPES, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Protein L purified -
Purification notes
Purified from hybridoma cell culture supernatant by Protein L affinity chromatography from fetal bovine serum containing medium (Protein L does not bind bovine IgG). -
Clonality
Monoclonal -
Clone number
5B3-6E3 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab219823 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.05 - 2 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 43 kDa).
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| Notes |
|---|
|
WB
Use a concentration of 0.05 - 2 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 43 kDa). |
Target
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Function
Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis. -
Involvement in disease
Defects in MT-CYB are a rare cause of mitochondrial dysfunction underlying different myopathies. They include mitochondrial encephalomyopathy, hypertrophic cardiomyopathy (HCM), and sporadic mitochondrial myopathy (MM). In mitochondrial myopathy, exercise intolerance is the predominant symptom. Additional features include lactic acidosis, muscle weakness and/or myoglobinuria. Defects in MTCYB are also found in cases of exercise intolerance accompanied by deafness, mental retardation, retinitis pigmentosa, cataract, growth retardation, epilepsy (multisystem disorder).
Cardiomyopathy, infantile histiocytoid
Leber hereditary optic neuropathy -
Sequence similarities
Belongs to the cytochrome b family. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 4519 Human
- SwissProt: P00156 Human
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Alternative names
- COB antibody
- Complex III subunit 3 antibody
- Complex III subunit III antibody
see all
Images
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Western blot - Anti-MT-CYB antibody [5B3-6E3] - N-terminal (ab219823)All lanes : Anti-MT-CYB antibody [5B3-6E3] - N-terminal (ab219823) at 0.1 µg/ml
Lane 1 : Mitochondria from cultured normal control human dermal fibroblasts neonatal (HDFn)
Lane 2 : Mitochondria from HDFn cells depleted of mtDNA by long-term proliferation in the presence of ethidium bromide
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labeled Goat-anti-mouse IgG
Developed using the ECL technique.
Predicted band size: 43 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?Mitochondrial proteins solubilized in 2% SDS were separated by SDS-PAGE and then transferred to PVDF membranes in CAPS buffer.
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![Western blot - Anti-MT-CYB antibody [5B3-6E3] - N-terminal (ab219823)](https://www.abcam.com/ps/products/219/ab219823/Images/ab219823-286098-anti-mt-cyb-antibody-5b3-6e3-n-terminal-western-blot.jpg)
Western blot - Anti-MT-CYB antibody [5B3-6E3] - N-terminal (ab219823)All lanes : Anti-MT-CYB antibody [5B3-6E3] - N-terminal (ab219823) at 2 µg/ml
Lane 1 : Whole cell extract of cultured normal control human dermal fibroblasts neonatal (HDFn)
Lane 2 : Whole cell extract HDFn-Rho0 cells depleted of mtDNA by long-term culture in the presence of ethidium bromide
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : HRP-labeled Goat-anti-mouse IgG
Developed using the ECL technique.
Predicted band size: 43 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?Proteins were separated by SDS-PAGE and then transferred to PVDF membranes in CAPS buffer.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab219823 has not yet been referenced specifically in any publications.