Overview

  • Product name

  • Description

    Rabbit polyclonal to MT-CYB
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cat
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (TPMRKINPLM KLINHSFIDL PTPSNISAWW NFGSLLGACL ILQITTGLFL) of Human MT-CYB (NP_536855).

  • Positive control

    • HeLa cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab81215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/1562500.

Target

  • Function

    Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.
  • Involvement in disease

    Defects in MT-CYB are a rare cause of mitochondrial dysfunction underlying different myopathies. They include mitochondrial encephalomyopathy, hypertrophic cardiomyopathy (HCM), and sporadic mitochondrial myopathy (MM). In mitochondrial myopathy, exercise intolerance is the predominant symptom. Additional features include lactic acidosis, muscle weakness and/or myoglobinuria. Defects in MTCYB are also found in cases of exercise intolerance accompanied by deafness, mental retardation, retinitis pigmentosa, cataract, growth retardation, epilepsy (multisystem disorder).
    Cardiomyopathy, infantile histiocytoid
    Leber hereditary optic neuropathy
  • Sequence similarities

    Belongs to the cytochrome b family.
  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • COB antibody
    • Complex III subunit 3 antibody
    • Complex III subunit III antibody
    • CYB_HUMAN antibody
    • CYTB antibody
    • Cytochrome b antibody
    • Cytochrome b c1 complex subunit 3 antibody
    • Cytochrome b-c1 complex subunit 3 antibody
    • Mitochondrially encoded cytochrome b antibody
    • MT-CYB antibody
    • MTCYB antibody
    • Ubiquinol cytochrome c reductase complex cytochrome b subunit antibody
    • Ubiquinol-cytochrome-c reductase complex cytochrome b subunit antibody
    see all

Images

  • Anti-MT-CYB antibody (ab81215) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 43 kDa
    Observed band size: 43 kDa



    Gel concentration: 12%

References

This product has been referenced in:

  • Maio N  et al. Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis. Haematologica N/A:N/A (2019). Read more (PubMed: 30765471) »
  • Maio N  et al. A Single Adaptable Cochaperone-Scaffold Complex Delivers Nascent Iron-Sulfur Clusters to Mammalian Respiratory Chain Complexes I-III. Cell Metab 25:945-953.e6 (2017). Read more (PubMed: 28380382) »
See all 2 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa wt vs HeLa rho-0)
Loading amount
70 µg
Specification
HeLa wt vs HeLa rho-0
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Jul 02 2012

Question
Answer

Thanks for your patience.  I have place the order for ab103405 (order#******), you should hopefully have it by the 20th Oct. If there is anything else I can do, just give me a shout. You have a great weekend too.

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Answer

Thank you forcontacting Abcam about ab81215, anti-MT-CYB antibody I am sorry to hear that you are experiencing problems with this antibody.  It is covered under our Abpromise, which guarantees that it will work on human tissue in western blot. I know that you have tried a few different variations but there I do have a few suggestions/questions: 1 - What type of tissue samples are you using? Do you use a positive control? 2 - It is recommenced to use this antibody at a concentration of 1ug/ml? Have you tried this concentration? 3 - What concentration of protein are you loading into your gel?  4 - Are you using detergent in your wash buffer? If so, is it Tween, Triton X-100? If you do use these detergents, lowering the concentration would help. I know that you have most probably tried variations of these suggestions but I would be grateful if you could let me know what you have tried, so that we can have it in our records. As I said previously, this antibody is covered under our Abpromise and so if we cannot resolve the issues with this particular vial, then I would be happy to send you a new vial from a different lot. I look forward to your reply.

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