Key features and details
- Rabbit polyclonal to MT-ND1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-MT-ND1 antibody
See all MT-ND1 primary antibodies
DescriptionRabbit polyclonal to MT-ND1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Chimpanzee, Gorilla
- WB: Mouse heart, liver, kidney, brain, skeletal muscle and stomach lysates. IHC: Human kidney tissue.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
- Mouse stomach normal tissue lysate - total protein (ab29256)
- Mouse liver tissue lysate - total protein (ab29301)
- Mouse kidney normal tissue lysate - total protein (ab29305)
- Mouse skeletal muscle tissue lysate - total protein (ab29711)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse heart normal tissue lysate - total protein (ab30291)
- Mouse brain tissue lysate - total protein (ab4022)
Our Abpromise guarantee covers the use of ab222892 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/500 - 1/5000. Predicted molecular weight: 36 kDa.|
FunctionCore subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Involvement in diseaseDefects in MT-ND1 are a cause of Leber hereditary optic neuropathy (LHON) [MIM:535000]. LHON is a maternally inherited disease resulting in acute or subacute loss of central vision, due to optic nerve dysfunction. Cardiac conduction defects and neurological defects have also been described in some patients. LHON results from primary mitochondrial DNA mutations affecting the respiratory chain complexes.
Defects in MT-ND1 are a cause of mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes syndrome (MELAS) [MIM:540000]. MELAS is a genetically heterogenious disorder, characterized by episodic vomiting, seizures, and recurrent cerebral insults resembling strokes and causing hemiparesis, hemianopsia, or cortical blindness.
Defects in MT-ND1 may be associated with susceptibility to Alzheimer disease mitochondrial (AD-MT) [MIM:502500]. Alzheimer disease is a neurodegenerative disorder characterized by progressive dementia, loss of cognitive abilities, and deposition of fibrillar amyloid proteins as intraneuronal neurofibrillary tangles, extracellular amyloid plaques and vascular amyloid deposits. The major constituent of these plaques is the neurotoxic amyloid-beta-APP 40-42 peptide (s), derived proteolytically from the transmembrane precursor protein APP by sequential secretase processing. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products such as C31 derived from APP, are also implicated in neuronal death.
Defects in MT-ND1 may be associated with non-insulin-dependent diabetes mellitus (NIDDM).
Sequence similaritiesBelongs to the complex I subunit 1 family.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- Complex I, subunit ND1 antibody
- Mitochondrially encoded NADH dehydrogenase 1 antibody
- MT-ND1 antibody
All lanes : Anti-MT-ND1 antibody (ab222892) at 1/500 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Mouse liver lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Mouse brain lysate
Lane 5 : Mouse skeletal muscle lysate
Lane 6 : Mouse stomach lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
Paraffin-embedded human kidney tissue stained for MT-ND1 using ab222892 at 1/100 dilution in immunohistochemical analysis.
ab222892 has been referenced in 1 publication.
- Hou X et al. Testosterone disruptor effect and gut microbiome perturbation in mice: Early life exposure to doxycycline. Chemosphere 222:722-731 (2019). PubMed: 30738315