Recombinant
RabMAb

Recombinant Anti-MT-ND1 antibody [EPR13466(2)] (ab181848)

Overview

  • Product name

    Anti-MT-ND1 antibody [EPR13466(2)]
    See all MT-ND1 primary antibodies
  • Description

    Rabbit monoclonal [EPR13466(2)] to MT-ND1
  • Host species

    Rabbit
  • Specificity

    This antibody is unsuitable for detecting cell line samples.

  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human MT-ND1 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P03886
    (Peptide available as ab195040)

  • Positive control

    • Human fetal heart and fetal muscle lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/20000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).Can be blocked with MT-ND1 peptide (ab195040).

This antibody is unsuitable for detecting cell line samples.

Target

  • Function

    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • Involvement in disease

    Defects in MT-ND1 are a cause of Leber hereditary optic neuropathy (LHON) [MIM:535000]. LHON is a maternally inherited disease resulting in acute or subacute loss of central vision, due to optic nerve dysfunction. Cardiac conduction defects and neurological defects have also been described in some patients. LHON results from primary mitochondrial DNA mutations affecting the respiratory chain complexes.
    Defects in MT-ND1 are a cause of mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes syndrome (MELAS) [MIM:540000]. MELAS is a genetically heterogenious disorder, characterized by episodic vomiting, seizures, and recurrent cerebral insults resembling strokes and causing hemiparesis, hemianopsia, or cortical blindness.
    Defects in MT-ND1 may be associated with susceptibility to Alzheimer disease mitochondrial (AD-MT) [MIM:502500]. Alzheimer disease is a neurodegenerative disorder characterized by progressive dementia, loss of cognitive abilities, and deposition of fibrillar amyloid proteins as intraneuronal neurofibrillary tangles, extracellular amyloid plaques and vascular amyloid deposits. The major constituent of these plaques is the neurotoxic amyloid-beta-APP 40-42 peptide (s), derived proteolytically from the transmembrane precursor protein APP by sequential secretase processing. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products such as C31 derived from APP, are also implicated in neuronal death.
    Defects in MT-ND1 may be associated with non-insulin-dependent diabetes mellitus (NIDDM).
  • Sequence similarities

    Belongs to the complex I subunit 1 family.
  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Complex I, subunit ND1 antibody
    • Mitochondrially encoded NADH dehydrogenase 1 antibody
    • MT-ND1 antibody
    • MTND1 antibody
    • NAD1 antibody
    • NADH dehydrogenase subunit 1 (complex I) antibody
    • NADH dehydrogenase subunit 1 antibody
    • NADH-ubiquinone oxidoreductase chain 1 antibody
    • NADH-ubiquinone oxidoreductase, subunit ND1 antibody
    • NADH1 antibody
    • ND1 antibody
    • NU1M_HUMAN antibody
    see all

Images

  • All lanes : Anti-MT-ND1 antibody [EPR13466(2)] (ab181848) at 1/5000 dilution (purified)

    Lane 1 : Human heart lysates
    Lane 2 : Mouse heart lysates
    Lane 3 : Rat heart lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 36 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • All lanes : Anti-MT-ND1 antibody [EPR13466(2)] (ab181848) at 1/20000 dilution (unpurified)

    Lane 1 : Human fetal heart lysate
    Lane 2 : Human fetal muscle lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 36 kDa

References

This product has been referenced in:

  • Ugun-Klusek A  et al. Monoamine oxidase-A promotes protective autophagy in human SH-SY5Y neuroblastoma cells through Bcl-2 phosphorylation. Redox Biol 20:167-181 (2019). Read more (PubMed: 30336354) »
  • Ishikawa K  et al. Concentration of mitochondrial DNA mutations by cytoplasmic transfer from platelets to cultured mouse cells. PLoS One 14:e0213283 (2019). Read more (PubMed: 30830936) »
See all 6 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (neuroblastoma)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
Specification
neuroblastoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Dec 18 2018

Application
Western blot
Sample
Rat Cell lysate - whole cell (Heart)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Aug 10 2016

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