Overview

  • Product name

  • Description

    Rabbit polyclonal to MT-ND3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human MT-ND3 aa 29-54.
    Sequence:

    GYIEKSTPYECGFDPISPARVPFSIK


    Database link: P03897

  • Positive control

    • Human rectum tissue, MCF7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab204977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • Involvement in disease

    Defects in MT-ND3 are a cause of Leigh syndrome (LS) [MIM:256000]. LS is a severe neurological disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions.
    Defects in MT-ND3 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease.
  • Sequence similarities

    Belongs to the complex I subunit 3 family.
  • Cellular localization

    Mitochondrion membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • EC=1.6.5.3 antibody
    • Mitochondrially encoded NADH dehydrogenase 3 antibody
    • MT-ND3 antibody
    • MTND3 antibody
    • NADH dehydrogenase subunit 3 antibody
    • NADH dehydrogenase, subunit 3 (complex I) antibody
    • NADH-ubiquinone oxidoreductase chain 3 antibody
    • ND3 antibody
    • NU3M_HUMAN antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human rectum tissue labeling MT-ND3 using ab204977 at 1/20 dilution.

  • Immunofluorescence analysis of MCF7 cells (Fixation/Permeabilization: PFA/Triton X-100) labeling MT-ND3 using ab204977 at 4 µg/mL.

References

ab204977 has not yet been referenced specifically in any publications.

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