Overview

  • Product name
    Anti-MT-ND3 antibody - N-terminal
    See all MT-ND3 primary antibodies
  • Description
    Rabbit polyclonal to MT-ND3 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MT-ND3 aa 9-39 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P03897

  • Positive control
    • K562 cell lysate, K562 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab170681 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/100 - 1/500. Predicted molecular weight: 13 kDa.

Target

  • Function
    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • Involvement in disease
    Defects in MT-ND3 are a cause of Leigh syndrome (LS) [MIM:256000]. LS is a severe neurological disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions.
    Defects in MT-ND3 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease.
  • Sequence similarities
    Belongs to the complex I subunit 3 family.
  • Cellular localization
    Mitochondrion membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC=1.6.5.3 antibody
    • Mitochondrially encoded NADH dehydrogenase 3 antibody
    • MT-ND3 antibody
    • MTND3 antibody
    • NADH dehydrogenase subunit 3 antibody
    • NADH dehydrogenase, subunit 3 (complex I) antibody
    • NADH-ubiquinone oxidoreductase chain 3 antibody
    • ND3 antibody
    • NU3M_HUMAN antibody
    see all

Images

  • Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram) labeling MT-ND3 with ab170681 at 1/10 dilution. FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

  • Anti-MT-ND3 antibody - N-terminal (ab170681) at 1/100 dilution + K562 cell lysate at 35 µg

    Predicted band size: 13 kDa

References

This product has been referenced in:
  • Zhou M  et al. A hypertension-associated mitochondrial DNA mutation introduces an m1G37 modification into tRNAMet, altering its structure and function. J Biol Chem 293:1425-1438 (2018). Read more (PubMed: 29222331) »
  • Saini SK  et al. DNA Methyltransferase1 (DNMT1) Isoform3 methylates mitochondrial genome and modulates its biology. Sci Rep 7:1525 (2017). WB ; Human . Read more (PubMed: 28484249) »
See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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