Key features and details
- Rabbit polyclonal to MT2-MMP
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MT2-MMP antibody
See all MT2-MMP primary antibodies
DescriptionRabbit polyclonal to MT2-MMP
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide within Mouse MT2-MMP aa 250-350. The exact sequence is proprietary.
Database link: O54732
- IHC-P: Human placenta tissue. ICC/IF: Jeg3 cells.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact email@example.com.
Previously labelled as MMP15.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab15475 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 76 kDa.|
1/50 for 10 min at RT. No special pretreatment is required for the immunohistochemical staining of formalin/paraffin tissues.
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionEndopeptidase that degrades various components of the extracellular matrix. May activate progelatinase A.
Tissue specificityAppeared to be synthesized preferentially in liver, placenta, testis, colon and intestine. Substantial amounts are also detected in pancreas, kidney, lung, heart and skeletal muscle.
Sequence similaritiesBelongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
modificationsThe precursor is cleaved by a furin endopeptidase.
- Information by UniProt
- AI503551 antibody
- Matrix metallopeptidase 15 membrane inserted antibody
- Matrix metalloproteinase 15 antibody
ICC/IF image of ab15475 stained Jeg3 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15475, 5g/ml) overnight at +4°C. The secondary antibody (green) was ab96899, Dylight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM
ab15475 staining MT2-MMP in human placenta by Immunohistochemistry (FFPE-sections).
ab15475 has been referenced in 2 publications.
- Wang H et al. Cryptotanshinone inhibits lung cancer invasion via microRNA-133a/matrix metalloproteinase 14 regulation. Oncol Lett 18:2554-2559 (2019). PubMed: 31452742
- Kuriyama S et al. LPP inhibits collective cell migration during lung cancer dissemination. Oncogene 35:952-64 (2016). WB . PubMed: 26028032