Recombinant Anti-MT4-MMP antibody [EP1270Y] (ab51075)

r these details. I am sorry this vial of antibody has not worked for this customer.

As requested, we are pleased to arrange an alternative free of charge replacement of ab39028. I can confirm that 1 vial of ab39028 has been added to your order number #####. This has Abcam order reference #####.

I would like to reassure you that this free of charge replacement vial is also covered by our Abpromise guarantee. Should the customer still be experiencing difficulties with the new vial, or if you have any further questions, please do not hesitate to let me know.

Thank you for your help and cooperation with this case. Please do not hesitate to contact me if you need anything further.

Thank you for your message and for providing this further information.

I am sorry there are no further suggestions to help improve the results on this occasion. I appreciate the time you have spent on these experiments and would be pleased to arrange a free of charge replacement or credit note in compensation. Alternatively, I am happy to provide a free of charge vial of a different antibody. Please let me know which one you would like to receive.

I look forward to hearing from you with details of how you would like to proceed.

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will provide us with vital information for our monitoring of product quality. I would like to reassure you that this antibody is tested and covered by our guarantee for IHC-P and human samples.

I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. Reviewing the details, I am sorry there not many tips to provide on this occasion to help improve the results. However, I can recommend you may like to consider trying the following options and considerations:

1. I can suggest to try less time for heat mediated antigen retrieval? This can sometimes require some optimization, and less time is sometimes better than more time. For example, try 5 minutes.

2. As MMP17 is a cell membrane protein, we would not recommend to permeabilize in Triton. Triton is a strong detergent which can disrupt membrane proteins. It can sometimes significantly affect the staining of membrane targets.

3. Is the IHC kit working well with other primary antibodies?

Alternatively, or if these tips do not work, I am pleased to offer you a free of charge replacement or credit note in compensation if the item has been purchased in the 6 month guarantee period. In this case I would appreciate if you are able to confirm the order number.

Thank you for your cooperation. I look forward to hearing from you with the results of the suggestions details of how you would like to proceed.

Thank you for your inquiry.

The concentration of ab51075 is lot dependent. The lot that we have in stock at the moment and of the last vial you receivedhas a concentration of 0.224 mg/mL.

I also would like to comment on the seemingly low concentration:

The IgG produced by rabbits have greater affinity and greater sensitivity than mouse IgG (about 100-1000 fold) and at the same time the total amount of IgG produced by the rabbit hybridomas is generally lower than in mouse hybridomas.

Many 'veteran' antibody users are generally familiar with the IgG amount provided from mouse antibodies and can judge how many reactions they may get or need to use based on concentration. Unfortunately, this experience is not transferable to rabbit monoclonal antibodies because the concentration scales are generally very different.

Since the specificity and sensitivity is much higher with rabbit monoclonal antibodies, less IgG is required per reaction.

I would like to reassure you that while the amount of IgG may not be comparable to mouse monoclonal antibodies, customers are receiving a comparable number of reactions/tests/blots/stainings per tube.

I hope this information is helpful. Please do not hesitate to contact me with any further questions in this matter and have a good weekend too.

Vielen Dank für Ihren Anruf gestern.

Ich habe jetzt genauere Information zum Immunogen erhalten:

Es liegt zwischen den Aminosäuren AA190 und 230 (SwissProt ID Q9ULZ9).

Ich hoffe, diese Information hilft Ihnen herauszufinden ob die zusätzliche Bande auf dem WB dem "ge-sheddeten" Teilstück des Proteins entspricht.

Thank you for your enquiry. We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage instructions on the datasheet for the antibody you purchased. These conditions will vary among our antibodies, therefore, it is important to verify the storage conditions for each of our products when you receive them. We guarantee all of our products to work for at least six month from the date of purchase when stored correctly. For more information on antibody storage and stability, please visit our Antibody Storage Guide on our Protocols and Troubleshooting Tips page (https://www.abcam.com/index.html?pageconfig=popular_protocols). I hope this information helps. Please do not hesitate to contact us if you need anything further.

Thank you for your inquiry. I am happy to confirm that the concentration of lot GR19885 is 0.224 mg/mL. I also would like to comment on the seemingly low concentration: The IgG produced by rabbits have greater affinity and greater sensitivity than mouse IgG (about 100-1000 fold) and at the same time the total amount of IgG produced by the rabbit hybridomas is generally lower than in mouse hybridomas. Many 'veteran' antibody users are generally familiar with the IgG amount provided from mouse antibodies and can judge how many reactions they may get or need to use based on concentration. Unfortunately, this experience is not transferable to rabbit monoclonal antibodies because the concentration scales are generally very different. Since the specificity and sensitivity is much higher with rabbit monoclonal antibodies, less IgG is required per reaction. I would like to reassure you that while the amount of IgG may not be comparable to mouse monoclonal antibodies, customers are receiving a comparable number of reactions/tests/blots/stainings per tube. I hope this information is helpful. Please do not hesitate to contact me with any further questions in this matter.