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Hi, I purchased EP1270Y – MMP17 Monoclonal [EP1270Y] antibody - but the vial does not have the concentration on it (it just says ‘tissue culture super’) and the data sheet just says Concentration: 100µl at mg/ml. Would you be able to tell me the concentration of this antibody? The Lot # is GR19885. Thank you,
Asked on Dec 08 2011
Thank you for your inquiry. I am happy to confirm that the concentration of lot GR19885 is 0.224 mg/mL. I also would like to comment on the seemingly low concentration: The IgG produced by rabbits have greater affinity and greater sensitivity than mouse IgG (about 100-1000 fold) and at the same time the total amount of IgG produced by the rabbit hybridomas is generally lower than in mouse hybridomas. Many 'veteran' antibody users are generally familiar with the IgG amount provided from mouse antibodies and can judge how many reactions they may get or need to use based on concentration. Unfortunately, this experience is not transferable to rabbit monoclonal antibodies because the concentration scales are generally very different. Since the specificity and sensitivity is much higher with rabbit monoclonal antibodies, less IgG is required per reaction. I would like to reassure you that while the amount of IgG may not be comparable to mouse monoclonal antibodies, customers are receiving a comparable number of reactions/tests/blots/stainings per tube. I hope this information is helpful. Please do not hesitate to contact me with any further questions in this matter.
Answered on Dec 08 2011