For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Dear technical support
Would you be able to advise me on the concentration as per ab51075 (anti-MMP17). The datasheet sheet does not specify the concentration.
Looking forward to your response.
In the meantime, have a nice weekend J
Asked on May 11 2012
Thank you for your inquiry.
The concentration of ab51075 is lot dependent. The lot that we have in stock at the moment and of the last vial you receivedhas a concentration of 0.224 mg/mL.
I also would like to comment on the seemingly low concentration:
The IgG produced by rabbits have greater affinity and greater sensitivity than mouse IgG (about 100-1000 fold) and at the same time the total amount of IgG produced by the rabbit hybridomas is generally lower than in mouse hybridomas.
Many 'veteran' antibody users are generally familiar with the IgG amount provided from mouse antibodies and can judge how many reactions they may get or need to use based on concentration. Unfortunately, this experience is not transferable to rabbit monoclonal antibodies because the concentration scales are generally very different.
Since the specificity and sensitivity is much higher with rabbit monoclonal antibodies, less IgG is required per reaction.
I would like to reassure you that while the amount of IgG may not be comparable to mouse monoclonal antibodies, customers are receiving a comparable number of reactions/tests/blots/stainings per tube.
I hope this information is helpful. Please do not hesitate to contact me with any further questions in this matter and have a good weekend too.
Answered on May 11 2012