Recombinant
RabMAb

Recombinant Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (ab247310)

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Overview

  • Product name

    Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free
    See all MT4-MMP primary antibodies

  • Description

    Rabbit monoclonal [EP1270Y] to MT4-MMP - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IF, WB, IHC-FoFrmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human MT4-MMP aa 150-250 (internal sequence). The exact sequence is proprietary.

  • General notes

    Ab247310 is the carrier-free version of ab51075. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab247310 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab247310 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 58 kDa (predicted molecular weight: 67 kDa).
IHC-FoFr Use at an assay dependent concentration. PubMed: 22022509
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    • Function

      Endopeptidase that degrades various components of the extracellular matrix, such as fibrin. May be involved in the activation of membrane-bound precursors of growth factors or inflammatory mediators, such as tumor necrosis factor-alpha. May also be involved in tumoral process. Not obvious if able to proteolytically activate progelatinase A. Does not hydrolyze collagen types I, II, III, IV and V, gelatin, fibronectin, laminin, decorin nor alpha1-antitrypsin.

    • Tissue specificity

      Expressed in brain, leukocytes, colon, ovary testis and breast cancer. Expressed also in many transformed and non-transformed cell types.

    • Sequence similarities

      Belongs to the peptidase M10A family.
      Contains 4 hemopexin-like domains.

    • Domain

      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

    • Post-translational
      modifications

      The precursor is cleaved by a furin endopeptidase.

    • Cellular localization

      Cell membrane. Secreted > extracellular space > extracellular matrix.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Matrix metalloproteinase 17 antibody
      • Matrix metalloproteinase 17 membrane inserted antibody
      • Matrix metalloproteinase-17 antibody
      • Membrane type 4 matrix metalloproteinase antibody
      • Membrane type matrix metalloproteinase 4 antibody
      • Membrane-type matrix metalloproteinase 4 antibody
      • Membrane-type-4 matrix metalloproteinase antibody
      • MMP 17 antibody
      • MMP-17 antibody
      • Mmp17 antibody
      • MMP17_HUMAN antibody
      • MT MMP 4 antibody
      • MT MMP4 antibody
      • MT-MMP 4 antibody
      • MT4 MMP antibody
      • MT4-MMP antibody
      • MT4MMP antibody
      • MTMMP 4 antibody
      • MTMMP4 antibody
      see all

    References

    ab247310 has not yet been referenced specifically in any publications.

    Publishing research using ab247310? Please let us know so that we can cite the reference in this datasheet.

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