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    mt4-mmp-antibody-ep1270y-bsa-and-azide-free-ab247310.pdf

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Cardiovascular Angiogenesis Adhesion / ECM Matrix Metalloproteinases MMP
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RecombinantRabMAb

Recombinant Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (ab247310)

  • Datasheet
  • Certificate of Compliance
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Western blot - Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (ab247310)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (ab247310)
  • Immunocytochemistry - Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (ab247310)
  • Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (ab247310)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1270Y] to MT4-MMP - BSA and Azide free
  • Suitable for: IHC-P, ICC, WB
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

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Human MMP9 peptide (ab73733)

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Overview

  • Product name

    Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free
    See all MT4-MMP primary antibodies
  • Description

    Rabbit monoclonal [EP1270Y] to MT4-MMP - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC, WBmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247310 is the carrier-free version of ab51075.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1270Y
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Adhesion / ECM
    • Matrix Metalloproteinases
    • MMP
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Enzymes
    • MMP
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • ECM enzymes
    • MMPs
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • MMPs
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Metalloprotease
    • MMPs

Associated products

  • Alternative Versions

    • Anti-MT4-MMP antibody [EP1270Y] (ab51075)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab247310 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 58 kDa (predicted molecular weight: 67 kDa).
Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 58 kDa (predicted molecular weight: 67 kDa).
Application notes
Is unsuitable for Flow Cyt or IP.

Target

  • Function

    Endopeptidase that degrades various components of the extracellular matrix, such as fibrin. May be involved in the activation of membrane-bound precursors of growth factors or inflammatory mediators, such as tumor necrosis factor-alpha. May also be involved in tumoral process. Not obvious if able to proteolytically activate progelatinase A. Does not hydrolyze collagen types I, II, III, IV and V, gelatin, fibronectin, laminin, decorin nor alpha1-antitrypsin.
  • Tissue specificity

    Expressed in brain, leukocytes, colon, ovary testis and breast cancer. Expressed also in many transformed and non-transformed cell types.
  • Sequence similarities

    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • Domain

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications

    The precursor is cleaved by a furin endopeptidase.
  • Cellular localization

    Cell membrane. Secreted > extracellular space > extracellular matrix.
  • Target information above from: UniProt accession Q9ULZ9 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4326 Human
    • Entrez Gene: 23948 Mouse
    • Entrez Gene: 288626 Rat
    • Omim: 602285 Human
    • SwissProt: Q9ULZ9 Human
    • SwissProt: Q9R0S3 Mouse
    • Unigene: 709245 Human
    • Unigene: 42047 Mouse
    • Alternative names

      • Matrix metalloproteinase 17 antibody
      • Matrix metalloproteinase 17 membrane inserted antibody
      • Matrix metalloproteinase-17 antibody
      • Membrane type 4 matrix metalloproteinase antibody
      • Membrane type matrix metalloproteinase 4 antibody
      • Membrane-type matrix metalloproteinase 4 antibody
      • Membrane-type-4 matrix metalloproteinase antibody
      • MMP 17 antibody
      • MMP-17 antibody
      • Mmp17 antibody
      • MMP17_HUMAN antibody
      • MT MMP 4 antibody
      • MT MMP4 antibody
      • MT-MMP 4 antibody
      • MT4 MMP antibody
      • MT4-MMP antibody
      • MT4MMP antibody
      • MTMMP 4 antibody
      • MTMMP4 antibody
      see all

    Images

    • Western blot - Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (ab247310)
      Western blot - Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (ab247310)
      Anti-MT4-MMP antibody [EP1270Y] (ab51075) at 1/1000 dilution + Mouse brain tissue lysate at 10 µg

      Secondary
      Goat anti-Rabbit HRP labeled at 1/2000 dilution

      Predicted band size: 67 kDa
      Observed band size: 58 kDa why is the actual band size different from the predicted?



      This data was developed using ab51075, the same antibody clone in a different buffer formulation.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (ab247310)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (ab247310)
      This data was developed using ab51075, the same antibody clone in a different buffer formulation.ab51075 (1:50) staining human MT4-MMP in human lymphoma tissue by immunohistochemistry using paraffin embedded tissue. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    • Immunocytochemistry - Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (ab247310)
      Immunocytochemistry - Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (ab247310)
      This data was developed using ab51075, the same antibody clone in a different buffer formulation.Immunofluorescent staining of HeLa cells using ab51075 (1:50).
    • Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (ab247310)
      Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (ab247310)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab247310? Please let us know so that we can cite the reference in this datasheet.

    ab247310 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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