Recombinant Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (ab247310)
![Western blot - Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (ab247310)](https://www.abcam.com/ps/products/247/ab247310/Images/ab247310-407871-anti-mt4-mmp-antibody-ep1270y-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (ab247310)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1270Y] to MT4-MMP - BSA and Azide free
- Suitable for: IHC-P, ICC, WB
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free
See all MT4-MMP primary antibodies -
Description
Rabbit monoclonal [EP1270Y] to MT4-MMP - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC, WBmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab247310 is the carrier-free version of ab51075.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1270Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab247310 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
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| ICC |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration. Detects a band of approximately 58 kDa (predicted molecular weight: 67 kDa).
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| Notes |
|---|
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. Detects a band of approximately 58 kDa (predicted molecular weight: 67 kDa). |
Target
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Function
Endopeptidase that degrades various components of the extracellular matrix, such as fibrin. May be involved in the activation of membrane-bound precursors of growth factors or inflammatory mediators, such as tumor necrosis factor-alpha. May also be involved in tumoral process. Not obvious if able to proteolytically activate progelatinase A. Does not hydrolyze collagen types I, II, III, IV and V, gelatin, fibronectin, laminin, decorin nor alpha1-antitrypsin. -
Tissue specificity
Expressed in brain, leukocytes, colon, ovary testis and breast cancer. Expressed also in many transformed and non-transformed cell types. -
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Post-translational
modificationsThe precursor is cleaved by a furin endopeptidase. -
Cellular localization
Cell membrane. Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 4326 Human
- Entrez Gene: 23948 Mouse
- Entrez Gene: 288626 Rat
- Omim: 602285 Human
- SwissProt: Q9ULZ9 Human
- SwissProt: Q9R0S3 Mouse
- Unigene: 709245 Human
- Unigene: 42047 Mouse
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Alternative names
- Matrix metalloproteinase 17 antibody
- Matrix metalloproteinase 17 membrane inserted antibody
- Matrix metalloproteinase-17 antibody
see all
Images
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Western blot - Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (ab247310)Anti-MT4-MMP antibody [EP1270Y] (ab51075) at 1/1000 dilution + Mouse brain tissue lysate at 10 µg
Secondary
Goat anti-Rabbit HRP labeled at 1/2000 dilution
Predicted band size: 67 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?This data was developed using ab51075, the same antibody clone in a different buffer formulation.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (ab247310)](https://www.abcam.com/ps/products/247/ab247310/Images/ab247310-2-anti-mt4-mmp-antibody-ep1270y-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (ab247310)This data was developed using ab51075, the same antibody clone in a different buffer formulation.ab51075 (1:50) staining human MT4-MMP in human lymphoma tissue by immunohistochemistry using paraffin embedded tissue. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Immunocytochemistry - Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (ab247310)](https://www.abcam.com/ps/products/247/ab247310/Images/ab247310-1-anti-mt4-mmp-antibody-ep1270y-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (ab247310) -
![Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (ab247310)](https://www.abcam.com/ps/products/247/ab247310/Images/ab247310-4-benefits-of-recombinant-antibodies.png)
Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (ab247310)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab247310 has not yet been referenced specifically in any publications.
