Overview

  • Product name
  • Description
    Rabbit polyclonal to MTA2
  • Host species
    Rabbit
  • Specificity
    No cross-reactivity to MTA1.
  • Tested applications
    Suitable for: WB, IHC-Fr, IHC-P, ICC/IF, ELISA, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:

    PAPSHPASTNEPIVLED

    , corresponding to amino acids 652 - 668 of Human MTA 2.

  • Positive control
    • HeLa cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab8106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).Can be blocked with Human MTA2 peptide (ab6243).
IHC-Fr Use at an assay dependent concentration. PubMed: 18413351
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 10 µg/ml.
ELISA Use at an assay dependent concentration.
IP Use a concentration of 5 µg/ml.

Target

  • Relevance
    The p53 tumor suppressor gene integrates numerous signals that control cell life and death. There are several proteins that are involved in the p53 pathway, including Chk2, p53R2, p53AIP1, Noxa, PIDD, and MTA2. The transcriptional activity of p53 is modulated by protein stability and acetylation. MTA2, also termed MTA1L1, was found to be a subunit of nucleosome remodeling and deacetylating (NuRD) complex. MTA2 modulates the enzymatic activity of the histone deacetylase complex and its expression reduces the levels of acetylated p53. Deacetylation of p53 by MTA2 represses p53 dependent transcriptional activation and modulates p53 mediated cell growth arrest and apoptosis. MTA2 is ubiquitously expressed in human tissues.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • DKFZp686F2281 antibody
    • Mata1l1 antibody
    • Metastasis associated 1 family member 2 antibody
    • Metastasis associated 1 like 1 antibody
    • Metastasis associated gene 1 like 1 antibody
    • Metastasis associated gene family member 2 antibody
    • Metastasis associated protein 2 antibody
    • Metastasis associated protein MTA 2 antibody
    • Metastasis associated protein MTA2 antibody
    • Mmta2 antibody
    • MTA1 L1 protein antibody
    • MTA1L1 antibody
    • p53 target protein in deacetylase complex antibody
    • PID antibody
    see all

Images

  • All lanes : Anti-MTA2 antibody (ab8106) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate with absence of blocking peptide
    Lane 2 : HeLa whole cell lysate with presence of blocking peptide

    Predicted band size: 75 kDa

  • ab8106 at 10µg/ml staining MTA2 in Hela cells by ICC/IF

  • ab8106 (2µg/ml) staining MTA2 in human ileum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • MTA2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to MTA2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab8106.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 77kDa: MTA2
  • Immunofluorescence of PID in HeLa cells using ab8106 at 10 ug/ml.

References

This product has been referenced in:
  • Campbell AE  et al. NuRD and CAF-1-mediated silencing of the D4Z4 array is modulated by DUX4-induced MBD3L proteins. Elife 7:N/A (2018). Read more (PubMed: 29533181) »
  • Christov CP  et al. A NuRD Complex from Xenopus laevis Eggs Is Essential for DNA Replication during Early Embryogenesis. Cell Rep 22:2265-2278 (2018). Read more (PubMed: 29490265) »
See all 18 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (Whole cell-lysate of HeLa and 293T cells in RIPA b)
Gel Running Conditions
Reduced Denaturing (10% precast Bis-Tris gel)
Loading amount
30 µg
Specification
Whole cell-lysate of HeLa and 293T cells in RIPA b
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Verified customer

Submitted May 24 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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