Recombinant
RabMAb

Anti-MTA2 antibody [EPR8537(2)] (ab171073)

Overview

  • Product name
    Anti-MTA2 antibody [EPR8537(2)]
    See all MTA2 primary antibodies
  • Description
    Rabbit monoclonal [EPR8537(2)] to MTA2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IHC-P, WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human MTA2 aa 650 to the C-terminus. The exact sequence is proprietary.
    Database link: O94776

  • Positive control
    • MCF7, HeLa and SW480 cell lysates. Paraffin-embedded Human kidney and Human colonic carcinoma tissue. HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab171073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250 - 1/500.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100.

Antigen retrieval is recommended

WB 1/1000 - 1/5000. Predicted molecular weight: 75 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Relevance
      The p53 tumor suppressor gene integrates numerous signals that control cell life and death. There are several proteins that are involved in the p53 pathway, including Chk2, p53R2, p53AIP1, Noxa, PIDD, and MTA2. The transcriptional activity of p53 is modulated by protein stability and acetylation. MTA2, also termed MTA1L1, was found to be a subunit of nucleosome remodeling and deacetylating (NuRD) complex. MTA2 modulates the enzymatic activity of the histone deacetylase complex and its expression reduces the levels of acetylated p53. Deacetylation of p53 by MTA2 represses p53 dependent transcriptional activation and modulates p53 mediated cell growth arrest and apoptosis. MTA2 is ubiquitously expressed in human tissues.
    • Cellular localization
      Nuclear
    • Database links
    • Alternative names
      • DKFZp686F2281 antibody
      • Mata1l1 antibody
      • Metastasis associated 1 family member 2 antibody
      • Metastasis associated 1 like 1 antibody
      • Metastasis associated gene 1 like 1 antibody
      • Metastasis associated gene family member 2 antibody
      • Metastasis associated protein 2 antibody
      • Metastasis associated protein MTA 2 antibody
      • Metastasis associated protein MTA2 antibody
      • Mmta2 antibody
      • MTA1 L1 protein antibody
      • MTA1L1 antibody
      • p53 target protein in deacetylase complex antibody
      • PID antibody
      see all

    Images

    • All lanes : Anti-MTA2 antibody [EPR8537(2)] (ab171073) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : SW480 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 75 kDa

    • Immunohistochemical analysis of paraffin-embedded Human colonic carcinoma tissue labeling MTA2 with ab171073 at 1/50 dilution. 

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling MTA2 with ab171073 at 1/50 dilution.

    • Immunofluorescent staining of HeLa cells labeling MTA2 with ab171073 at 1/250 dilution.

    • Flow cytometric analysis of permeabilized Hela cells labeling MTA2 with ab171073 at 1/100 dilution (red) or a rabbit IgG negative (green).

    References

    ab171073 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab171073.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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