Product nameAnti-MTA2 antibody [EPR8537(2)]
See all MTA2 primary antibodies
DescriptionRabbit monoclonal [EPR8537(2)] to MTA2
Tested applicationsSuitable for: ICC/IF, Flow Cyt, IHC-P, WBmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human MTA2 aa 650 to the C-terminus. The exact sequence is proprietary.
Database link: O94776
- MCF7, HeLa and SW480 cell lysates. Paraffin-embedded Human kidney and Human colonic carcinoma tissue. HeLa cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab171073 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/250 - 1/500.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/50 - 1/100.
Antigen retrieval is recommended
|WB||1/1000 - 1/5000. Predicted molecular weight: 75 kDa.|
RelevanceThe p53 tumor suppressor gene integrates numerous signals that control cell life and death. There are several proteins that are involved in the p53 pathway, including Chk2, p53R2, p53AIP1, Noxa, PIDD, and MTA2. The transcriptional activity of p53 is modulated by protein stability and acetylation. MTA2, also termed MTA1L1, was found to be a subunit of nucleosome remodeling and deacetylating (NuRD) complex. MTA2 modulates the enzymatic activity of the histone deacetylase complex and its expression reduces the levels of acetylated p53. Deacetylation of p53 by MTA2 represses p53 dependent transcriptional activation and modulates p53 mediated cell growth arrest and apoptosis. MTA2 is ubiquitously expressed in human tissues.
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All lanes : Anti-MTA2 antibody [EPR8537(2)] (ab171073) at 1/1000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : SW480 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 75 kDa
Immunohistochemical analysis of paraffin-embedded Human colonic carcinoma tissue labeling MTA2 with ab171073 at 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling MTA2 with ab171073 at 1/50 dilution.
Immunofluorescent staining of HeLa cells labeling MTA2 with ab171073 at 1/250 dilution.
Flow cytometric analysis of permeabilized Hela cells labeling MTA2 with ab171073 at 1/100 dilution (red) or a rabbit IgG negative (green).
ab171073 has not yet been referenced specifically in any publications.