Anti-MTA2/PID antibody (ab8106)
Key features and details
- Rabbit polyclonal to MTA2/PID
- Suitable for: IHC-Fr, ELISA, WB, IHC-P, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-MTA2/PID antibody
See all MTA2/PID primary antibodies -
Description
Rabbit polyclonal to MTA2/PID -
Host species
Rabbit -
Specificity
No cross-reactivity to MTA1. -
Tested applications
Suitable for: IHC-Fr, ELISA, WB, IHC-P, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8106 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use at an assay dependent concentration. PubMed: 18413351
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ELISA |
Use at an assay dependent concentration.
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WB | (1) |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
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IHC-P |
Use at an assay dependent concentration.
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ICC/IF |
Use a concentration of 10 µg/ml.
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IP | (1) |
Use at an assay dependent concentration.
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Notes |
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IHC-Fr
Use at an assay dependent concentration. PubMed: 18413351 |
ELISA
Use at an assay dependent concentration. |
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa). |
IHC-P
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 10 µg/ml. |
IP
Use at an assay dependent concentration. |
Target
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Function
May be involved in the regulation of gene expression as repressor and activator. The repression might be related to covalent modification of histone proteins. -
Tissue specificity
Widely expressed. -
Sequence similarities
Contains 1 BAH domain.
Contains 1 ELM2 domain.
Contains 1 GATA-type zinc finger.
Contains 1 SANT domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 9219 Human
- Entrez Gene: 23942 Mouse
- Entrez Gene: 361724 Rat
- Omim: 603947 Human
- SwissProt: O94776 Human
- SwissProt: Q9R190 Mouse
- Unigene: 173043 Human
- Unigene: 25339 Mouse
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Alternative names
- DKFZp686F2281 antibody
- Mata1l1 antibody
- Metastasis associated 1 family member 2 antibody
see all
Images
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All lanes : Anti-MTA2/PID antibody (ab8106) at 1 µg/ml
Lane 1 : HeLa whole cell lysate with absence of blocking peptide
Lane 2 : HeLa whole cell lysate with presence of blocking peptide
Predicted band size: 75 kDa -
ab8106 at 10µg/ml staining MTA2/PID in Hela cells by ICC/IF
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ab8106 (2µg/ml) staining MTA2/PID in human ileum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
Immunofluorescence of PID in HeLa cells using ab8106 at 10 ug/ml.
Protocols
Datasheets and documents
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Datasheet download
References (31)
ab8106 has been referenced in 31 publications.
- Wang W et al. Zinc-finger protein CXXC5 promotes breast carcinogenesis by regulating the TSC1/mTOR signaling pathway. J Biol Chem 299:102812 (2023). PubMed: 36539038
- Deng P et al. RAD21 amplification epigenetically suppresses interferon signaling to promote immune evasion in ovarian cancer. J Clin Invest 132:N/A (2022). PubMed: 36201246
- Apipongrat D et al. Serum proteomic profiling reveals MTA2 and AGO2 as potential prognostic biomarkers associated with disease activity and adverse outcomes in multiple myeloma. PLoS One 17:e0278464 (2022). PubMed: 36454786
- Vinjamur DS et al. ZNF410 represses fetal globin by singular control of CHD4. Nat Genet 53:719-728 (2021). PubMed: 33859416
- Shi J et al. MTA2 sensitizes gastric cancer cells to PARP inhibition by induction of DNA replication stress. Transl Oncol 14:101167 (2021). PubMed: 34280886