Overview

  • Product name

  • Description

    Rabbit polyclonal to MTA2/PID
  • Host species

    Rabbit
  • Specificity

    No cross-reactivity to MTA1.
  • Tested applications

    Suitable for: WB, IHC-Fr, IHC-P, ICC/IF, ELISA, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human MTA2/PID aa 652-668.
    Sequence:

    PAPSHPASTNEPIVLED


    (Peptide available as ab6243)

  • Positive control

    • HeLa cell lysate
  • General notes

     This product was previously labelled as MTA2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab8106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
IHC-Fr Use at an assay dependent concentration. PubMed: 18413351
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 10 µg/ml.
ELISA Use at an assay dependent concentration.
IP Use a concentration of 5 µg/ml.

Target

  • Function

    May be involved in the regulation of gene expression as repressor and activator. The repression might be related to covalent modification of histone proteins.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Contains 1 BAH domain.
    Contains 1 ELM2 domain.
    Contains 1 GATA-type zinc finger.
    Contains 1 SANT domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp686F2281 antibody
    • Mata1l1 antibody
    • Metastasis associated 1 family member 2 antibody
    • Metastasis associated 1 like 1 antibody
    • Metastasis associated gene 1 like 1 antibody
    • Metastasis associated gene family member 2 antibody
    • Metastasis associated protein 2 antibody
    • Metastasis associated protein MTA 2 antibody
    • Metastasis associated protein MTA2 antibody
    • Metastasis-associated 1-like 1 antibody
    • Metastasis-associated protein MTA2 antibody
    • Mmta2 antibody
    • MTA1 L1 protein antibody
    • MTA1-L1 protein antibody
    • MTA1L1 antibody
    • MTA2 antibody
    • MTA2_HUMAN antibody
    • p53 target protein in deacetylase complex antibody
    • PID antibody
    see all

Images

  • All lanes : Anti-MTA2/PID antibody (ab8106) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate with absence of blocking peptide
    Lane 2 : HeLa whole cell lysate with presence of blocking peptide

    Predicted band size: 75 kDa

  • ab8106 at 10µg/ml staining MTA2/PID in Hela cells by ICC/IF

  • ab8106 (2µg/ml) staining MTA2/PID in human ileum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

  • MTA2/PID was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to MTA2/PID and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab8106.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 77kDa: MTA2/PID

  • Immunofluorescence of PID in HeLa cells using ab8106 at 10 ug/ml.

References

This product has been referenced in:

  • Campbell AE  et al. NuRD and CAF-1-mediated silencing of the D4Z4 array is modulated by DUX4-induced MBD3L proteins. Elife 7:N/A (2018). Read more (PubMed: 29533181) »
  • Christov CP  et al. A NuRD Complex from Xenopus laevis Eggs Is Essential for DNA Replication during Early Embryogenesis. Cell Rep 22:2265-2278 (2018). Read more (PubMed: 29490265) »
See all 19 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (Whole cell-lysate of HeLa and 293T cells in RIPA b)
Gel Running Conditions
Reduced Denaturing (10% precast Bis-Tris gel)
Loading amount
30 µg
Specification
Whole cell-lysate of HeLa and 293T cells in RIPA b
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Verified customer

Submitted May 24 2018

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