• Product name

  • Description

    Rabbit polyclonal to MTA2/PID
  • Host species

  • Specificity

    No cross-reactivity to MTA1.
  • Tested applications

    Suitable for: WB, IHC-Fr, IHC-P, ICC/IF, ELISA, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human MTA2/PID aa 652-668.


    (Peptide available as ab6243)

  • Positive control

    • HeLa cell lysate
  • General notes

     This product was previously labelled as MTA2




Our Abpromise guarantee covers the use of ab8106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
IHC-Fr Use at an assay dependent concentration. PubMed: 18413351
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 10 µg/ml.
ELISA Use at an assay dependent concentration.
IP Use a concentration of 5 µg/ml.


  • Function

    May be involved in the regulation of gene expression as repressor and activator. The repression might be related to covalent modification of histone proteins.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Contains 1 BAH domain.
    Contains 1 ELM2 domain.
    Contains 1 GATA-type zinc finger.
    Contains 1 SANT domain.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp686F2281 antibody
    • Mata1l1 antibody
    • Metastasis associated 1 family member 2 antibody
    • Metastasis associated 1 like 1 antibody
    • Metastasis associated gene 1 like 1 antibody
    • Metastasis associated gene family member 2 antibody
    • Metastasis associated protein 2 antibody
    • Metastasis associated protein MTA 2 antibody
    • Metastasis associated protein MTA2 antibody
    • Metastasis-associated 1-like 1 antibody
    • Metastasis-associated protein MTA2 antibody
    • Mmta2 antibody
    • MTA1 L1 protein antibody
    • MTA1-L1 protein antibody
    • MTA1L1 antibody
    • MTA2 antibody
    • MTA2_HUMAN antibody
    • p53 target protein in deacetylase complex antibody
    • PID antibody
    see all


  • All lanes : Anti-MTA2/PID antibody (ab8106) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate with absence of blocking peptide
    Lane 2 : HeLa whole cell lysate with presence of blocking peptide

    Predicted band size: 75 kDa

  • ab8106 at 10µg/ml staining MTA2/PID in Hela cells by ICC/IF

  • ab8106 (2µg/ml) staining MTA2/PID in human ileum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

  • MTA2/PID was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to MTA2/PID and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab8106.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 77kDa: MTA2/PID

  • Immunofluorescence of PID in HeLa cells using ab8106 at 10 ug/ml.


This product has been referenced in:

  • Campbell AE  et al. NuRD and CAF-1-mediated silencing of the D4Z4 array is modulated by DUX4-induced MBD3L proteins. Elife 7:N/A (2018). Read more (PubMed: 29533181) »
  • Christov CP  et al. A NuRD Complex from Xenopus laevis Eggs Is Essential for DNA Replication during Early Embryogenesis. Cell Rep 22:2265-2278 (2018). Read more (PubMed: 29490265) »
See all 19 Publications for this product

Customer reviews and Q&As

Western blot
Human Cell lysate - whole cell (Whole cell-lysate of HeLa and 293T cells in RIPA b)
Gel Running Conditions
Reduced Denaturing (10% precast Bis-Tris gel)
Loading amount
30 µg
Whole cell-lysate of HeLa and 293T cells in RIPA b
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Submitted May 24 2018

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