Overview

  • Product name
  • Description
    Mouse monoclonal to MTAP
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-155 of Human MTAP

Properties

Applications

Our Abpromise guarantee covers the use of ab55517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 31 kDa.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine.
  • Tissue specificity
    Ubiquitously expressed.
  • Sequence similarities
    Belongs to the PNP/MTAP phosphorylase family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5' methylthioadenosine phosphorylase antibody
    • 5''-methylthioadenosine phosphorylase antibody
    • BDMF antibody
    • c86fus antibody
    • DMSFH antibody
    • DMSMFH antibody
    • Epididymis luminal protein 249 antibody
    • HEL 249 antibody
    • LGMBF antibody
    • MeSAdo phosphorylase antibody
    • Methylthioadenosine phosphorylase antibody
    • MSAP antibody
    • MTA phosphorylase antibody
    • MTAP antibody
    • MTAP_HUMAN antibody
    • MTAPase antibody
    • S methyl 5 thioadenosine phosphorylase antibody
    • S methyl 5' thioadenosine phosphorylase antibody
    • S-methyl-5''-thioadenosine phosphorylase antibody
    see all

Images

  • MTAP antibody (ab55517) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ab55517 at 10 ug/ml staining MTAP in human Hella cells by Immunocytochemistry/ Immunofluorescence.

  • Overlay histogram showing HeLa cells stained with ab55517 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55517, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.

References

This product has been referenced in:
  • Limm K  et al. Characterization of the Methylthioadenosine Phosphorylase Polymorphism rs7023954 - Incidence and Effects on Enzymatic Function in Malignant Melanoma. PLoS One 11:e0160348 (2016). WB . Read more (PubMed: 27479139) »
  • Woollard WJ  et al. Independent Loss of Methylthioadenosine Phosphorylase (MTAP) in Primary Cutaneous T-Cell Lymphoma. J Invest Dermatol 136:1238-46 (2016). Read more (PubMed: 26872600) »
See all 3 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab55517.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up