Recombinant
RabMAb

Recombinant Anti-MTAP antibody [EPR6892] (ab126623)

Overview

  • Product name

    Anti-MTAP antibody [EPR6892]
    See all MTAP primary antibodies
  • Description

    Rabbit monoclonal [EPR6892] to MTAP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human MTAP (Q13126).

  • Positive control

    • Human heart, HeLa, HT29, Rat C6, Mouse RAW 264.7 and NIH 3T3 cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab126623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 31 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      Plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine.
    • Tissue specificity

      Ubiquitously expressed.
    • Sequence similarities

      Belongs to the PNP/MTAP phosphorylase family.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • 5' methylthioadenosine phosphorylase antibody
      • 5''-methylthioadenosine phosphorylase antibody
      • BDMF antibody
      • c86fus antibody
      • DMSFH antibody
      • DMSMFH antibody
      • Epididymis luminal protein 249 antibody
      • HEL 249 antibody
      • LGMBF antibody
      • MeSAdo phosphorylase antibody
      • Methylthioadenosine phosphorylase antibody
      • MSAP antibody
      • MTA phosphorylase antibody
      • MTAP antibody
      • MTAP_HUMAN antibody
      • MTAPase antibody
      • S methyl 5 thioadenosine phosphorylase antibody
      • S methyl 5' thioadenosine phosphorylase antibody
      • S-methyl-5''-thioadenosine phosphorylase antibody
      see all

    Images

    • All lanes : Anti-MTAP antibody [EPR6892] (ab126623) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Human heart lysate
      Lane 3 : C6 cell lysate
      Lane 4 : RAW 264.7 cell lysate
      Lane 5 : NIH 3T3 cell lysate
      Lane 6 : HT29 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 31 kDa

    References

    ab126623 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    This product is known to not work in this application or species.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Xenografted tumor intercranial)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate
    Permeabilization
    No
    Specification
    Xenografted tumor intercranial
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C
    Fixative
    Formaldehyde

    Mr. Anton Poral

    Verified customer

    Submitted Jul 02 2019

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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