Recombinant
RabMAb

Recombinant Anti-MTAP antibody [EPR6893] (Phycoerythrin) (ab225149)

Overview

  • Product name

    Anti-MTAP antibody [EPR6893] (Phycoerythrin)
    See all MTAP primary antibodies
  • Description

    Rabbit monoclonal [EPR6893] to MTAP (Phycoerythrin)
  • Host species

    Rabbit
  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human MTAP aa 200-300. The exact sequence is proprietary.
    Database link: Q13126

  • Positive control

    • Flow Cyt: HeLa cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab225149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/1000.

The cellular localisation of this product has been verified in ICC/IF.

Target

  • Function

    Plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the PNP/MTAP phosphorylase family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • 5' methylthioadenosine phosphorylase antibody
    • 5''-methylthioadenosine phosphorylase antibody
    • BDMF antibody
    • c86fus antibody
    • DMSFH antibody
    • DMSMFH antibody
    • Epididymis luminal protein 249 antibody
    • HEL 249 antibody
    • LGMBF antibody
    • MeSAdo phosphorylase antibody
    • Methylthioadenosine phosphorylase antibody
    • MSAP antibody
    • MTA phosphorylase antibody
    • MTAP antibody
    • MTAP_HUMAN antibody
    • MTAPase antibody
    • S methyl 5 thioadenosine phosphorylase antibody
    • S methyl 5' thioadenosine phosphorylase antibody
    • S-methyl-5''-thioadenosine phosphorylase antibody
    see all

Images

  • Overlay histogram showing HeLa cells stained with ab225149 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225149, 1/1000 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

    This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

References

ab225149 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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