Anti-MTBP antibody (ab115529)
Key features and details
- Rabbit polyclonal to MTBP
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MTBP antibody -
Description
Rabbit polyclonal to MTBP -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow, Pig -
Immunogen
Synthetic peptide:
GAVECFEEEDSNSRESLS
conjugated to KLH, corresponding to amino acids 122-139 of Human MTBP (NP_071328.2). -
Positive control
- Human thymus and tonsil tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab115529 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Inhibits cell migration in vitro and suppresses the invasive behavior of tumor cells (By similarity). May play a role in MDM2-dependent p53/TP53 homeostasis in unstressed cells. Inhibits autoubiquitination of MDM2, thereby enhancing MDM2 stability. This promotes MDM2-mediated ubiquitination of p53/TP53 and its subsequent degradation. -
Sequence similarities
Belongs to the MTBP family. - Information by UniProt
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Database links
- Entrez Gene: 27085 Human
- Entrez Gene: 105837 Mouse
- Omim: 605927 Human
- SwissProt: Q96DY7 Human
- SwissProt: Q80VU3 Mouse
- SwissProt: Q8BJS8 Mouse
- SwissProt: Q8BTU9 Mouse
- SwissProt: Q8BTW0 Mouse
see all -
Alternative names
- hMTBP antibody
- MDM 2 binding protein antibody
- MDM 2 BP antibody
see all
Images
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ab115529 at 10ug/ml staining MTBP in Formalin-fixed, Paraffin-embedded Human thymus tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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ab115529 at 10ug/ml staining MTBP in Formalin-fixed, Paraffin-embedded Human tonsil tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Datasheets and documents
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Datasheet download
References (3)
ab115529 has been referenced in 3 publications.
- Shayimu P et al. MTBP promoted the proliferation, migration and invasion of colon cancer cells by activating the expression of ZEB2. Anim Cells Syst (Seoul) 25:152-160 (2021). PubMed: 34262658
- Song Y et al. MTBP regulates cell survival and therapeutic sensitivity in TP53 wildtype glioblastomas. Theranostics 9:6019-6030 (2019). PubMed: 31534534
- Wang W et al. MDM2 binding protein as a predictor of metastasis and a novel prognostic biomarker in patients with gastric cancer. Oncol Lett 14:6409-6416 (2017). PubMed: 29422956