Overview

  • Product name

    Anti-MTCO1 antibody [11D8B7]
    See all MTCO1 primary antibodies
  • Description

    Mouse monoclonal [11D8B7] to MTCO1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Saccharomyces cerevisiae
    Does not react with: Mouse, Rat, Human
  • Immunogen

    Full length protein. This information is considered to be commercially sensitive.

  • Positive control

    • Mitochondria from yeast membrane extract
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab110270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 3 µg/ml. Predicted molecular weight: 57 kDa.

Target

  • Function

    Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B.
  • Pathway

    Energy metabolism; oxidative phosphorylation.
  • Involvement in disease

    Defects in MT-CO1 are a cause of Leber hereditary optic neuropathy (LHON) [MIM:535000]. LHON is a maternally inherited disease resulting in acute or subacute loss of central vision, due to optic nerve dysfunction. Cardiac conduction defects and neurological defects have also been described in some patients. LHON results from primary mitochondrial DNA mutations affecting the respiratory chain complexes.
    Defects in MT-CO1 are a cause of anemia sideroblastic acquired idiopathic (AISA) [MIM:516030]; a disease characterized by inadequate formation of heme and excessive accumulation of iron in mitochondria.
    Defects in MT-CO1 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome.
    Defects in MT-CO1 are associated with recurrent myoglobinuria mitochondrial (RM-MT) [MIM:550500]. Recurrent myoglobinuria is characterized by recurrent attacks of rhabdomyolysis (necrosis or disintegration of skeletal muscle) associated with muscle pain and weakness, and followed by excretion of myoglobin in the urine.
    Defects in MT-CO1 are a cause of deafness sensorineural mitochondrial (DFNM) [MIM:500008]. DFNM is a form of non-syndromic deafness with maternal inheritance. Affected individuals manifest progressive, postlingual, sensorineural hearing loss involving high frequencies.
    Defects in MT-CO1 are a cause of colorectal cancer (CRC) [MIM:114500].
  • Sequence similarities

    Belongs to the heme-copper respiratory oxidase family.
  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

    • Alternative names

      • COI antibody
      • COX I antibody
      • COX1 antibody
      • COX1_HUMAN antibody
      • COXI antibody
      • Cytochrome c oxidase polypeptide I antibody
      • Cytochrome c oxidase subunit 1 antibody
      • Cytochrome C Oxidase subunit I antibody
      • Mitochondrially encoded cytochrome c oxidase I antibody
      • MT CO1 antibody
      • MT-CO1 antibody
      • MTCO 1 antibody
      • MTCO1 antibody
      see all

    Images

    • Anti-MTCO1 antibody [11D8B7] (ab110270) at 3 µg/ml + Mitochondria from yeast membrane extract

      Predicted band size: 57 kDa

    References

    This product has been referenced in:

    • Taylor NG  et al. The Assembly Factor Pet117 Couples Heme a Synthase Activity to Cytochrome Oxidase Assembly. J Biol Chem 292:1815-1825 (2017). Read more (PubMed: 27998984) »
    • Swenson S  et al. Analysis of Oligomerization Properties of Heme a Synthase Provides Insights into Its Function in Eukaryotes. J Biol Chem 291:10411-25 (2016). Read more (PubMed: 26940873) »
    See all 8 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Application
    Western blot
    Sample
    Cryptococcus neoformans Cell lysate - other (Subcellular fractionations)
    Gel Running Conditions
    Reduced Denaturing (Criterion TGX 4-20%)
    Loading amount
    40 µg
    Specification
    Subcellular fractionations
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Jan 30 2018

    Answer

    I am sorry for the delay in getting back to you.

    I have now checked the other antibodies we have against this target and unfortunately none would be predicted to cross react with the Drosophila protein except the rabbit polyclonal ab90668. However there have been some problem in the production of this antibody and we are currently not expecting it to be back in stock until Spring 2013.

    I am sorry that I could not be of more help in this instance. You may be interested to review the antibodies we have which have shown cross reactivity with Drosophila proteins:

    https://www.abcam.com/index.html?pageconfig=resource&rid=11526&pid=7

    If I can be of any further assistance, please do not hesitate to let me know.

    Read More

    Answer

    Thank you for contacting us. These antibodies are indeed raised in mouse and these antibodies will work better in human, mouse, rat cells and tissue sections. In case you are planning to use these antibodies on mouse tissue sections then we would recommend following additional blocking steps as given in the attached document https://www.abcam.com/index.html?pageconfig=resource&rid=11465 We do have anti MTCO1 antibodies that are raised in Rabbit. The catalogue numbers are ab45918; https://www.abcam.com/MTCO1-antibody-Mitochondrial-Marker-ab45918.html And ab90668; https://www.abcam.com/MTCO1-antibody-ab90668.html I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

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