Anti-MTCO1 antibody [5D11-1C9] - N-terminal (ab219824)
Key features and details
- Mouse monoclonal [5D11-1C9] to MTCO1 - N-terminal
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2a
Get better batch-to-batch reproducibility with a recombinant antibody
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Overview
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Product name
Anti-MTCO1 antibody [5D11-1C9] - N-terminal -
Description
Mouse monoclonal [5D11-1C9] to MTCO1 - N-terminal -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human MTCO1 (N terminal).
Database link: P00395 -
Positive control
- WB: Mitochondria from cultured normal control human dermal fibroblasts neonatal (HDFn); Whole cell extract of cultured normal control human dermal fibroblasts neonatal (HDFn).
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General notes
Western blot advice:
Hydrophobic intrinsic membrane proteins such as the core mtDNA-encoded proteins of the mitochondrial OXPHOS complexes tend to run faster in SDS-PAGE than predicted by their amino acid composition. This is likely due to incomplete unfolding of the protein and a more negative charge:mass ratio.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 0.36% HEPES, 0.87% Sodium chloride -
Concentration information loading...
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Purification notes
Purified from hybridoma cell culture supernatant by Protein L affinity chromatography from fetal bovine serum containing medium (Protein L does not bind bovine IgG). -
Clonality
Monoclonal -
Clone number
5D11-1C9 -
Isotype
IgG2a -
Light chain type
kappa
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab219824 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.2 - 2 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 57 kDa).
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Notes |
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WB
Use a concentration of 0.2 - 2 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 57 kDa). |
Images
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All lanes : Anti-MTCO1 antibody [5D11-1C9] - N-terminal (ab219824) at 0.1 µg/ml
Lane 1 : Mitochondria from cultured normal control human dermal fibroblasts neonatal (HDFn)
Lane 2 : Mitochondria from HDFn cells depleted of mtDNA by long-term proliferation in the presence of ethidium bromide
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labeled Goat-anti-mouse IgG
Developed using the ECL technique.
Predicted band size: 57 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?Mitochondrial proteins solubilized in 2% SDS were separated by SDS-PAGE and then transferred to PVDF membranes in CAPS buffer.
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All lanes : Anti-MTCO1 antibody [5D11-1C9] - N-terminal (ab219824) at 2 µg/ml
Lane 1 : Whole cell extract of cultured normal control human dermal fibroblasts neonatal (HDFn)
Lane 2 : Whole cell extract HDFn-Rho0 cells depleted of mtDNA by long-term culture in the presence of ethidium bromide
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : HRP-labeled Goat-anti-mouse IgG
Developed using the ECL technique.
Predicted band size: 57 kDaProteins were separated by SDS-PAGE and then transferred to PVDF membranes in CAPS buffer.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab219824 has not yet been referenced specifically in any publications.