Key features and details
- Mouse monoclonal [12C4F12] to MTCO2
- Suitable for: IHC-Fr, WB, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
Product nameAnti-MTCO2 antibody [12C4F12]
See all MTCO2 primary antibodies
DescriptionMouse monoclonal [12C4F12] to MTCO2
Tested applicationsSuitable for: IHC-Fr, WB, ICC/IF, Flow Cytmore details
Species reactivityReacts with: Human
Full length native protein (purified). This information is considered to be commercially sensitive.
- WB: Mitochondrial lysate from human heart tissue. ICC/IF: Cultured human embryonic lung derived fibroblasts. Flow Cyt: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: HEPES buffered saline
Concentration information loading...
Purification notesab110258 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. ab110258 was judged near homogeneity by SDS-PAGE.
Light chain typekappa
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab110258 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa.|
|ICC/IF||Use a concentration of 5 - 10 µg/ml. (heat-induced antigen-retrieval improves signal)|
|Flow Cyt||Use a concentration of 1 µg/ml.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
FunctionCytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.
Involvement in diseaseDefects in MT-CO2 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome.
Sequence similaritiesBelongs to the cytochrome c oxidase subunit 2 family.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- COII antibody
- COX 2 antibody
- COX II antibody
Anti-MTCO2 antibody [12C4F12] (ab110258) at 1 µg/ml + Mitochondrial lysate from human heart tissue at 5 µg
Predicted band size: 26 kDa
Immunocytochemistry/Immunofluorescence analysis of human embryonic lung derived fibroblasts (MRC5) labelling Cytochrome C oxidase subunit II with ab110258 at 5 µg/ml. An Alexa Fluor® 488-conjugated goat anti-mouse IgG2a isotype specific secondary antibody was used at 2 µg/ml.
ab110258, at 1 µg/ml, staining Cytochrome C oxidase subunit II in HeLa cells by Flow Cytometry (Blue).
An isotype control antibody, at 1 µg/ml, staining in HeLa cells (Red).
ab110258 has been referenced in 158 publications.
- Lobo-Jarne T et al. Multiple pathways coordinate assembly of human mitochondrial complex IV and stabilization of respiratory supercomplexes. EMBO J N/A:e103912 (2020). PubMed: 32511785
- Li K et al. Maternally Inherited Diabetes Mellitus Associated with a Novel m.15897G>A Mutation in Mitochondrial tRNAThr Gene. J Diabetes Res 2020:2057187 (2020). PubMed: 32083134
- Nozawa T et al. TBC1D9 regulates TBK1 activation through Ca2+ signaling in selective autophagy. Nat Commun 11:770 (2020). PubMed: 32034138
- Protasoni M et al. Respiratory supercomplexes act as a platform for complex III-mediated maturation of human mitochondrial complexes I and IV. EMBO J 39:e102817 (2020). PubMed: 31912925
- Zhao X et al. Hypertension-associated mitochondrial DNA 4401A>G mutation caused the aberrant processing of tRNAMet, all 8 tRNAs and ND6 mRNA in the light-strand transcript. Nucleic Acids Res 47:10340-10356 (2019). PubMed: 31504769