Overview

  • Product name
    Anti-MTCO2 antibody [12C4F12]
    See all MTCO2 primary antibodies
  • Description
    Mouse monoclonal [12C4F12] to MTCO2
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) corresponding to Human MTCO2. Full length native Human Cytochrome C oxidase subunit II purified by SDS PAGE.

  • Positive control
    • WB: Isolated mitochondria from human heart. ICC/IF: Cultured human embryonic lung derived fibroblasts. Flow Cyt: HeLa cells.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab110258 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa.
ICC/IF Use a concentration of 5 - 10 µg/ml. (heat-induced antigen-retrieval improves signal)
Flow Cyt Use a concentration of 1 µg/ml.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.
  • Involvement in disease
    Defects in MT-CO2 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome.
  • Sequence similarities
    Belongs to the cytochrome c oxidase subunit 2 family.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • COII antibody
    • COX2 antibody
    • COX2_HUMAN antibody
    • COXII antibody
    • Cytochrome c oxidase II antibody
    • Cytochrome c oxidase polypeptide II antibody
    • Cytochrome c oxidase subunit 2 antibody
    • MT CO2 antibody
    • MT-CO2 antibody
    • MTCO2 antibody
    see all

Images

  • Anti-MTCO2 antibody [12C4F12] (ab110258) at 1 µg/ml + Human heart tissue lysate - mitochondrial extract (ab110337) at 5 µg

    Predicted band size: 26 kDa

  • ab110258 staining human lung sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab110258 at 1/250 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-mouse polyclonal antibody at 1/200 was used as the secondary antibody.

    See Abreview

  • Immunocytochemistry/Immunofluorescence analysis of human embryonic lung derived fibroblasts (MRC5) labelling Cytochrome C oxidase subunit II with ab110258 at 5 µg/ml. An Alexa Fluor® 488-conjugated goat anti-mouse IgG2a isotype specific secondary antibody was used at 2 µg/ml.

  • ab110258, at 1 µg/ml, staining Cytochrome C oxidase subunit II in HeLa cells by Flow Cytometry (Blue).

    An isotype control antibody, at 1 µg/ml, staining in HeLa cells (Red).

References

This product has been referenced in:
  • Peter B  et al. Defective mitochondrial protease LonP1 can cause classical mitochondrial disease. Hum Mol Genet 27:1743-1753 (2018). Read more (PubMed: 29518248) »
  • Wang L  et al. PTEN-L is a novel protein phosphatase for ubiquitin dephosphorylation to inhibit PINK1-Parkin-mediated mitophagy. Cell Res 28:787-802 (2018). Read more (PubMed: 29934616) »
See all 110 Publications for this product

Customer reviews and Q&As

1-10 of 20 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate ph 6
Permeabilization
No
Specification
brain tissue
Blocking step
Normal Goat Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
Fixative
Formaldehyde

Herr Dr. Markus Kipp

Verified customer

Submitted Aug 22 2018

Application
Western blot
Sample
Rat Tissue lysate - whole (Placenta)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
Specification
Placenta
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Jeremiah Gaudet

Verified customer

Submitted Mar 23 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Placenta)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
Specification
Placenta
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Jeremiah Gaudet

Verified customer

Submitted Mar 22 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS Cells)
Permeabilization
Yes - 0.2% NP-40
Specification
U2OS Cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Formaldehyde

Dr. Michael Munson

Verified customer

Submitted Sep 21 2017

Application
Western blot
Sample
Human Cell lysate - other (U2OS Cells - Cytosol v Membrane Fractionation)
Gel Running Conditions
Reduced Denaturing (4-20% Acrylamide)
Loading amount
30 µg
Specification
U2OS Cells - Cytosol v Membrane Fractionation
Blocking step
Casein as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C

Dr. Michael Munson

Verified customer

Submitted Sep 08 2017

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 4°C
Sample
Zebrafish Cell (Whole Body)
Specification
Whole Body
Permeabilization
Yes - Triton X-100
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Nov 25 2014

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Rat Cell lysate - whole cell (Primary Neurons)
Specification
Primary Neurons
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Aug 22 2014

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Zebrafish Tissue lysate - whole (Larvae 5hpf)
Specification
Larvae 5hpf
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Aug 22 2014

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (15% GEL)
Sample
Human Cell lysate - whole cell (H460)
Specification
H460
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C

Abcam user community

Verified customer

Submitted May 26 2014

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (15% GEL)
Sample
Mouse Cell lysate - whole cell (mouse Embryonic Fibroblasts)
Specification
mouse Embryonic Fibroblasts
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C

Abcam user community

Verified customer

Submitted May 22 2014

1-10 of 20 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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