Anti-MTCO2 antibody [12C4F12] (ab110258)

Mouse monoclonal MTCO2 antibody [12C4F12]. Validated in WB, IHC, Flow Cyt, ICC/IF and tested in Human. Cited in 111 publication(s). Independently reviewed in 14 review(s).

Overview

  • Product name

    Anti-MTCO2 antibody [12C4F12]
    See all MTCO2 primary antibodies
  • Description

    Mouse monoclonal [12C4F12] to MTCO2
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, IHC-Fr, WB, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length native protein (purified). This information is considered to be commercially sensitive.

  • Positive control

    • WB: Isolated mitochondria from human heart. ICC/IF: Cultured human embryonic lung derived fibroblasts. Flow Cyt: HeLa cells.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab110258 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa.
ICC/IF Use a concentration of 5 - 10 µg/ml. (heat-induced antigen-retrieval improves signal)
Flow Cyt Use a concentration of 1 µg/ml.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.
  • Involvement in disease

    Defects in MT-CO2 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome.
  • Sequence similarities

    Belongs to the cytochrome c oxidase subunit 2 family.
  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • COII antibody
    • COX 2 antibody
    • COX II antibody
    • COX2 antibody
    • COX2_HUMAN antibody
    • COXII antibody
    • Cytochrome c oxidase II antibody
    • Cytochrome c oxidase polypeptide II antibody
    • Cytochrome c oxidase subunit 2 antibody
    • MT CO2 antibody
    • MT-CO2 antibody
    • MTCO2 antibody
    see all

Images

  • Anti-MTCO2 antibody [12C4F12] (ab110258) at 1 µg/ml + Human heart tissue lysate - mitochondrial extract (ab110337) at 5 µg

    Predicted band size: 26 kDa

  • ab110258 staining human lung sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab110258 at 1/250 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-mouse polyclonal antibody at 1/200 was used as the secondary antibody.

    See Abreview

  • Immunocytochemistry/Immunofluorescence analysis of human embryonic lung derived fibroblasts (MRC5) labelling Cytochrome C oxidase subunit II with ab110258 at 5 µg/ml. An Alexa Fluor® 488-conjugated goat anti-mouse IgG2a isotype specific secondary antibody was used at 2 µg/ml.

  • ab110258, at 1 µg/ml, staining Cytochrome C oxidase subunit II in HeLa cells by Flow Cytometry (Blue).

    An isotype control antibody, at 1 µg/ml, staining in HeLa cells (Red).

References

This product has been referenced in:

  • Gentric G  et al. PML-Regulated Mitochondrial Metabolism Enhances Chemosensitivity in Human Ovarian Cancers. Cell Metab 29:156-173.e10 (2019). Read more (PubMed: 30244973) »
  • Signes A  et al. APOPT1/COA8 assists COX assembly and is oppositely regulated by UPS and ROS. EMBO Mol Med 11:N/A (2019). Read more (PubMed: 30552096) »
See all 141 Publications for this product

Customer reviews and Q&As

1-6 of 6 Q&A

Question

I would like to know if the epitopes recognized by the following antibodies are known:
Code
Name
https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-12C4F12--ab110258.html
https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-12C4F12--ab110258.html
https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-12C4F12--ab110258.html
Mouse monoclonal
https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-EPR3314-ab79393.html
https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-EPR3314-ab79393.html
https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-EPR3314-ab79393.html
Rabbit monoclonal
https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-4B12A5-ab110271.html
https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-4B12A5-ab110271.html
https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-4B12A5-ab110271.html
Mouse monoclonal
https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-ab91317.html
https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-ab91317.html
Rabbit polyclonal
https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-EPR3313-ab109739.html
https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-EPR3313-ab109739.html
https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-EPR3313-ab109739.html
Rabbit monoclonal
https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-ab101802.html
https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-ab101802.html
Rabbit polyclonal
Awaiting your kind reply, I remain

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Answer

Thank you for contacting us.

The immunogens are as follows;

ab79393; A synthetic peptide corresponding to residues in human Cytochrome C oxidase subunit II
ab109739; A synthetic peptide corresponding to residues in Human Cytochrome C oxidase subunit II.
ab101802; A synthetic peptide corresponding to residues in Human Cytochrome C oxidase subunit II.
ab91317; A synthetic peptide corresponding to residues in Human Cytochrome C oxidase subunit II.
ab110258: exact epitope is not determined. Antigen was full length protein
ab110271: exact epitope is not determined. Antigen was full length protein

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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https://www.abcam.com/abreviews

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Answer

Thank your for your inquiry.


Unfortunately, I am unable to answer your questions. This precise epitope of this antibody was not mapped by our lab. We currently also do not know of anyone that did map the binding site of this antibody.

I am sorry that could not be of more help on this occasion and hope this information was nevertheless helpful. Please do not hesitate to contact me again with any further questions in this matter.

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Answer

Thanks so much for the additional details. Your protocol and sample preparation seems fine. The bands on the western blot in your data are a bit lower than the band on the example blot on our datasheet. However I don't think this necessarily means the antibody is not detecting the correct protein. Slight variations in apparent molecular weight are common and can be caused many things such as differences in acrylamide gels, voltage/amps used to run the gel, etc. In addition, other antibodies to Cytochrome C oxidase subunit II on our website show a migration pattern very similar to that in your data. For examples of this please see the online datasheets for ab91317 and ab101802. I hope this is helpful. Please contact me if you have any further questions or concerns.

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Answer

Thank you for your enquiry and for including your western blot data. To assist you most efficiently in troubleshooting your western blot data, please complete the following questionnaire. Thanks in advance for the additional information. ===================== 1) Abcam product code ab 2) Abcam order reference number or product batch number 3) Description of the problem 4) Sample preparation: Type of sample (whole cell lysates, fraction, recombinant protein…): Lysis buffer Protease inhibitors: Phosphatase inhibitors Reducing agent Boiling for ≥5 min? yes/no Protein loaded ug/lane or cells/lane 5) Positive control 6) Negative control 7) Primary antibody (If more than one was used, describe in “additional notes”) : Concentration or dilution Diluent buffer Incubation time Incubation temperature: 8) Secondary antibody: Species: Reacts against: Concentration or dilution Diluent buffer Incubation time Incubation temperature: Fluorochrome or enzyme conjugate: Please send this questionnaire by e-mail by cutting and pasting the text in your e-mail (no attachments other than images) to technical@abcam.com.

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Answer

Thank you for contacting Abcam. I have placed the discount code for testing ab110258 in IHC below, the code is under Anne-Emilie DeCleves, as she will be the one writing the Abreview but when you come to use the code you yourself will be able to use it, when placing an order. We do normally like to issue the code before the order is placed and I have made an exception in this case but in future if you wish to take advantage of the testing discount, please wait until the code is issued before placing the order. This is so that we can keep track of it accurately in our system. If there is anything else I can do, please let me know. Testing Discount Details DISCOUNT CODE: ****** Expiration date: ******** I am very pleased to hear you would like to accept our offer and test ab110258 in IHC. This code will give you 1 free[PRIMARY ANTIBODYbefore the expiration date. To redeem this offer, please submit an Abreview for IHC and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.  

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Answer

Thank you very much for your interest in ab110258. To our knowledge, ab110258 has not been tested in IHC. Therefore, I can offer a discount off a future purchase if you buy ab110258  now, test it in IHC and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab110258 in IHC. I will then send a discount code. This code must be issued before purchasing ab110258 so please wait for my reply before ordering. 2. Purchase ab110258 either by phone, fax, or online (www.abcam.com). 3. Test it in  IHC. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODYordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab110258 turns out to be unsuitable for IHC, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.  

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