Application
Western blot
Sample
Candida albicans Cell lysate - other (Cytosol and mitochondrial fraction)
Gel Running Conditions
Reduced Denaturing (4-12% precast gel)
Loading amount
15 µg
Treatment
Induced with 10 mM proline for 1 h
Specification
Cytosol and mitochondrial fraction
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBST
Dilution
1/2000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Supersignal WestDura
Negative control
Cytosolic fraction devoid of Put2-3xHA signal (mitochondrial protein).
Exposure
30 second(s)
Positive control
Mitochondrial fraction with crisp Put2-3xHA signal. Put2 is a mitochondrial localized protein in Candida albicans.
Additional data
Additional Notes
I tested this antibody together with ab110326 a-Por1 (VDAC)
Abcam response
Thank you for your valuable feedback. This antibody has been tested with Saccharomyces cerevisiae and the sequence homology with Candida albican is really low, therefore this antibody was not recommended with this specie.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Fitz Gerald S Silao
Verified customer
Submitted Apr 26 2021