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RecombinantRabMAb

Recombinant Anti-MTCO2 antibody [EPR3313] (ab109739)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (1)

Product price, shipping and contact information

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Abpromise

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Find out more.

Western blot - Anti-MTCO2 antibody [EPR3313] (ab109739)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO2 antibody [EPR3313] (ab109739)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO2 antibody [EPR3313] (ab109739)
  • Anti-MTCO2 antibody [EPR3313] (ab109739)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3313] to MTCO2
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-MTCO2 antibody [EPR3313]
    See all MTCO2 primary antibodies
  • Description

    Rabbit monoclonal [EPR3313] to MTCO2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MTCO2 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • HeLa, K562, MCF7, and THP1 cell lysates; Human kidney and liver tissues.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.01% BSA, 9.85% Tris glycine
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR3313
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Oxidative phosphorylation
    • Complex IV
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Cytochromes
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Alternative Versions

    • Anti-MTCO2 antibody [EPR3313] - BSA and Azide free (ab247921)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

Our Abpromise guarantee covers the use of ab109739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Antigen retrieval recommended; heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function

      Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.
    • Involvement in disease

      Defects in MT-CO2 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome.
    • Sequence similarities

      Belongs to the cytochrome c oxidase subunit 2 family.
    • Cellular localization

      Mitochondrion inner membrane.
    • Target information above from: UniProt accession P00403 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 4513 Human
      • Omim: 516040 Human
      • SwissProt: P00403 Human
      • Alternative names

        • COII antibody
        • COX 2 antibody
        • COX II antibody
        • COX2 antibody
        • COX2_HUMAN antibody
        • COXII antibody
        • Cytochrome c oxidase II antibody
        • Cytochrome c oxidase polypeptide II antibody
        • Cytochrome c oxidase subunit 2 antibody
        • MT CO2 antibody
        • MT-CO2 antibody
        • MTCO2 antibody
        see all

      Images

      • Western blot - Anti-MTCO2 antibody [EPR3313] (ab109739)
        Western blot - Anti-MTCO2 antibody [EPR3313] (ab109739)
        All lanes : Anti-MTCO2 antibody [EPR3313] (ab109739) at 1/1000 dilution

        Lane 1 : HeLa cell lysate
        Lane 2 : K562 cell lysate
        Lane 3 : MCF7 cell lysate
        Lane 4 : THP1 cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 26 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO2 antibody [EPR3313] (ab109739)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO2 antibody [EPR3313] (ab109739)

        ab109739 at 1/100 dilution staining Cytochrome C oxidase subunit II in paraffin-embedded Human kidney tissue by Immunohistochemistry.

        Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO2 antibody [EPR3313] (ab109739)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO2 antibody [EPR3313] (ab109739)

        ab109739 at 1/100 dilution staining Cytochrome C oxidase subunit II in paraffin-embedded Human liver tissue by Immunohistochemistry.

        Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

      • Anti-MTCO2 antibody [EPR3313] (ab109739)
        Anti-MTCO2 antibody [EPR3313] (ab109739)

      Protocols

      • Western blot protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (1)

      Publishing research using ab109739? Please let us know so that we can cite the reference in this datasheet.

      ab109739 has been referenced in 1 publication.

      • Ouyang Q  et al. Mutations in mitochondrial enzyme GPT2 cause metabolic dysfunction and neurological disease with developmental and progressive features. Proc Natl Acad Sci U S A 113:E5598-607 (2016). PubMed: 27601654

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-2 of 2 Abreviews or Q&A

      Question

      I would like to know if the epitopes recognized by the following antibodies are known:
      Code
      Name
      https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-12C4F12--ab110258.html
      https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-12C4F12--ab110258.html
      https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-12C4F12--ab110258.html
      Mouse monoclonal
      https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-EPR3314-ab79393.html
      https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-EPR3314-ab79393.html
      https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-EPR3314-ab79393.html
      Rabbit monoclonal
      https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-4B12A5-ab110271.html
      https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-4B12A5-ab110271.html
      https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-4B12A5-ab110271.html
      Mouse monoclonal
      https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-ab91317.html
      https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-ab91317.html
      Rabbit polyclonal
      https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-EPR3313-ab109739.html
      https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-EPR3313-ab109739.html
      https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-EPR3313-ab109739.html
      Rabbit monoclonal
      https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-ab101802.html
      https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-ab101802.html
      Rabbit polyclonal
      Awaiting your kind reply, I remain

      Read More

      Abcam community

      Verified customer

      Asked on Sep 21 2012

      Answer

      Thank you for contacting us.

      The immunogens are as follows;

      ab79393; A synthetic peptide corresponding to residues in human Cytochrome C oxidase subunit II
      ab109739; A synthetic peptide corresponding to residues in Human Cytochrome C oxidase subunit II.
      ab101802; A synthetic peptide corresponding to residues in Human Cytochrome C oxidase subunit II.
      ab91317; A synthetic peptide corresponding to residues in Human Cytochrome C oxidase subunit II.
      ab110258: exact epitope is not determined. Antigen was full length protein
      ab110271: exact epitope is not determined. Antigen was full length protein

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Abcam Scientific Support

      Answered on Sep 21 2012

      Western blot abreview for Anti-MTCO2 antibody [EPR3313]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (HeLa WT vs HeLa rho-0)
      Loading amount
      13 µg
      Specification
      HeLa WT vs HeLa rho-0
      Gel Running Conditions
      Reduced Denaturing (Reduced Denaturing (4-15%))
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
      Read More

      Abcam user community

      Verified customer

      Submitted Jul 27 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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