Recombinant Anti-MTCO2 antibody [EPR3313] (ab109739)
![Western blot - Anti-MTCO2 antibody [EPR3313] (ab109739)](https://www.abcam.com/ps/products/109/ab109739/Images/ab109739-122710-anti-mtco2-antibody-epr3313-western-blot.jpg "Western blot - Anti-MTCO2 antibody [EPR3313] (ab109739)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3313] to MTCO2
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-MTCO2 antibody [EPR3313]
See all MTCO2 primary antibodies -
Description
Rabbit monoclonal [EPR3313] to MTCO2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human MTCO2 aa 1-100. The exact sequence is proprietary.
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Positive control
- HeLa, K562, MCF7, and THP1 cell lysates; Human kidney and liver tissues.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.01% BSA, 9.85% Tris glycine -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3313 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab109739 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 26 kDa. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Antigen retrieval recommended; heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. |
Target
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Function
Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. -
Involvement in disease
Defects in MT-CO2 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome. -
Sequence similarities
Belongs to the cytochrome c oxidase subunit 2 family. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 4513 Human
- Omim: 516040 Human
- SwissProt: P00403 Human
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Alternative names
- COII antibody
- COX 2 antibody
- COX II antibody
see all
Images
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Western blot - Anti-MTCO2 antibody [EPR3313] (ab109739)All lanes : Anti-MTCO2 antibody [EPR3313] (ab109739) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : THP1 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 26 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO2 antibody [EPR3313] (ab109739)](https://www.abcam.com/ps/products/109/ab109739/Images/ab109739-122709-anti-mtco2-antibody-epr3313-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO2 antibody [EPR3313] (ab109739)ab109739 at 1/100 dilution staining Cytochrome C oxidase subunit II in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO2 antibody [EPR3313] (ab109739)](https://www.abcam.com/ps/products/109/ab109739/Images/ab109739-122708-anti-mtco2-antibody-epr3313-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO2 antibody [EPR3313] (ab109739)ab109739 at 1/100 dilution staining Cytochrome C oxidase subunit II in paraffin-embedded Human liver tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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![Anti-MTCO2 antibody [EPR3313] (ab109739)](https://www.abcam.com/ps/products/109/ab109739/Images/ab109739-4-benefits-of-recombinant-antibodies.png)
Anti-MTCO2 antibody [EPR3313] (ab109739)
Protocols
References (1)
ab109739 has been referenced in 1 publication.
- Ouyang Q et al. Mutations in mitochondrial enzyme GPT2 cause metabolic dysfunction and neurological disease with developmental and progressive features. Proc Natl Acad Sci U S A 113:E5598-607 (2016). PubMed: 27601654
