Anti-MTCO2 antibody [EPR3313] (ab109739)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-MTCO2 antibody [EPR3313]
See all MTCO2 primary antibodies -
DescriptionRabbit monoclonal [EPR3313] to MTCO2
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC -
Species reactivityReacts with: Human
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Immunogen
Synthetic peptide within Human MTCO2 aa 1-100. The exact sequence is proprietary.
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Positive control
- HeLa, K562, MCF7, and THP1 cell lysates; Human kidney and liver tissues.
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General notes
A trial size is available to purchase for this antibody.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 0.01% BSA, 9.85% Tris glycine -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR3313
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IsotypeIgG
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Research areas
Applications
Our Abpromise guarantee covers the use of ab109739 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 26 kDa. | |
| IP | 1/10 - 1/100. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Antigen retrieval recommended; heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. |
Target
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FunctionCytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.
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Involvement in diseaseDefects in MT-CO2 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome.
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Sequence similaritiesBelongs to the cytochrome c oxidase subunit 2 family.
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Cellular localizationMitochondrion inner membrane.
- Information by UniProt
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Database links
- Entrez Gene: 4513 Human
- Omim: 516040 Human
- SwissProt: P00403 Human
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Alternative names
- COII antibody
- COX2 antibody
- COX2_HUMAN antibody
see all
Images
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![Western blot - Anti-MTCO2 antibody [EPR3313] (ab109739)](https://www.abcam.com/ps/products/109/ab109739/Images/Cytochrome-C-oxidase-subunit-II-Primary-antibodies-ab109739-1.JPG)
Western blot - Anti-MTCO2 antibody [EPR3313] (ab109739)All lanes : Anti-MTCO2 antibody [EPR3313] (ab109739) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : THP1 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 26 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO2 antibody [EPR3313] (ab109739)](https://www.abcam.com/ps/products/109/ab109739/Images/Cytochrome-C-oxidase-subunit-II-Primary-antibodies-ab109739-2.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO2 antibody [EPR3313] (ab109739)ab109739 at 1/100 dilution staining Cytochrome C oxidase subunit II in paraffin-embedded Human kidney tissue by Immunohistochemistry. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO2 antibody [EPR3313] (ab109739)](https://www.abcam.com/ps/products/109/ab109739/Images/Cytochrome-C-oxidase-subunit-II-Primary-antibodies-ab109739-3.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO2 antibody [EPR3313] (ab109739)ab109739 at 1/100 dilution staining Cytochrome C oxidase subunit II in paraffin-embedded Human liver tissue by Immunohistochemistry.
References
This product has been referenced in:
- Ouyang Q et al. Mutations in mitochondrial enzyme GPT2 cause metabolic dysfunction and neurological disease with developmental and progressive features. Proc Natl Acad Sci U S A 113:E5598-607 (2016). Read more (PubMed: 27601654) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"