Recombinant
RabMAb

Recombinant Anti-MTCO2 antibody [EPR3313] (ab109739)

Overview

  • Product name

    Anti-MTCO2 antibody [EPR3313]
    See all MTCO2 primary antibodies
  • Description

    Rabbit monoclonal [EPR3313] to MTCO2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MTCO2 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • HeLa, K562, MCF7, and THP1 cell lysates; Human kidney and liver tissues.
  • General notes

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Antigen retrieval recommended; heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function

      Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.
    • Involvement in disease

      Defects in MT-CO2 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome.
    • Sequence similarities

      Belongs to the cytochrome c oxidase subunit 2 family.
    • Cellular localization

      Mitochondrion inner membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • COII antibody
      • COX 2 antibody
      • COX II antibody
      • COX2 antibody
      • COX2_HUMAN antibody
      • COXII antibody
      • Cytochrome c oxidase II antibody
      • Cytochrome c oxidase polypeptide II antibody
      • Cytochrome c oxidase subunit 2 antibody
      • MT CO2 antibody
      • MT-CO2 antibody
      • MTCO2 antibody
      see all

    Images

    • All lanes : Anti-MTCO2 antibody [EPR3313] (ab109739) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : THP1 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 26 kDa

    • ab109739 at 1/100 dilution staining Cytochrome C oxidase subunit II in paraffin-embedded Human kidney tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • ab109739 at 1/100 dilution staining Cytochrome C oxidase subunit II in paraffin-embedded Human liver tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Ouyang Q  et al. Mutations in mitochondrial enzyme GPT2 cause metabolic dysfunction and neurological disease with developmental and progressive features. Proc Natl Acad Sci U S A 113:E5598-607 (2016). Read more (PubMed: 27601654) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Filter by Application

    Filter by Species

    Filter by Ratings

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa WT vs HeLa rho-0)
    Loading amount
    13 µg
    Specification
    HeLa WT vs HeLa rho-0
    Gel Running Conditions
    Reduced Denaturing (Reduced Denaturing (4-15%))
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

    Abcam user community

    Verified customer

    Submitted Jul 27 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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