Overview

  • Product name
    Anti-MTCO2 antibody [MM7D-5A11CA9]
    See all MTCO2 primary antibodies
  • Description
    Mouse monoclonal [MM7D-5A11CA9] to MTCO2
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ICC, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Human, Monkey
    Does not react with: Mouse, Rat
  • Immunogen

    Human heart mitochondria.

  • Positive control
    • Human liver and heart mitochondrial extracts; Human heart homogenate; 143B(Human osteosarcoma), HeLa and COS7 cells.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab156031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 25.5 kDa).
ICC Use a concentration of 1 µg/ml.
Flow Cyt Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IP Use at an assay dependent concentration.

Target

  • Function
    Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.
  • Involvement in disease
    Defects in MT-CO2 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome.
  • Sequence similarities
    Belongs to the cytochrome c oxidase subunit 2 family.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • COII antibody
    • COX2 antibody
    • COX2_HUMAN antibody
    • COXII antibody
    • Cytochrome c oxidase II antibody
    • Cytochrome c oxidase polypeptide II antibody
    • Cytochrome c oxidase subunit 2 antibody
    • MT CO2 antibody
    • MT-CO2 antibody
    • MTCO2 antibody
    see all

Images

  • All lanes : Anti-MTCO2 antibody [MM7D-5A11CA9] (ab156031) at 5 µg/ml

    Lane 1 : Human Liver Mitochondria Extract
    Lane 2 : Human Heart Mitochondria Extrac

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - AP at 1/3000 dilution

    Predicted band size: 25.5 kDa



    1X Blocking Buffer (ab126587) in H2O was used for blocking and incubation steps.
  • Immunoprecipitation Coomassie stained gel for Anti-COXII antibody (ab156031).
    Lane 1: Ladder
    Lane 2: COXII Immunoprecipitated from 1mg Human Heart Homogenate (HHH) Extract. ab156031 anti-COXII captures the entire Cytochrome c oxidase complex in immunoprecipitation.
  • Immunocytochemical analysis of 143B (Human osteosarcoma) wild type (WT) and mtDNA lacking (?0) cells (paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min)) labeling COXII with ab156031 at 1µg/ml (red) and anti-HSP60 (ab46798) at 1/1000 (green) as a counter stain. Secondary: Alexa 594 goat anti-mouse IgG at 1/1000 and Alexa 488 goat anti-rat IgG at 1/1000. ab156031 shows good reactivity with the wild type (143B-WT) cell line, but shows no signal with the mtDNA lacking (143B-?0) cell line. Cytochrome c oxidase 2 is exclusively encoded by mitochondrial DNA. The anti-HSP60 counter stain antibody identified mitochondria in both cell lines.
  • Immunocytochemical analysis of COS7 (monkey kidney fibroblast) cells (paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min)) labeling COXII with ab156031 at 1µg/ml (red). Secondary: Alexa 594 goat anti-mouse IgG at 1/1000. ab156031 shows good reactivity with the COS7 cell type indicating cross reactivity with monkey cytochrome c oxidase.
  • Flow cytometric analysis of HeLa cells (fixed and permeabilized with methanol) labeling COXII with ab156031 (blue) at 1µg/ml and an equal amount of an isotype control antibody (red).

References

ab156031 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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