Overview

  • Product name
    Anti-MTCO3 antibody [DA5BC4]
  • Description
    Mouse monoclonal [DA5BC4] to MTCO3
  • Host species
    Mouse
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human, Saccharomyces cerevisiae
  • Immunogen

    Full length native Cytochrome C Oxidase subunit III (Saccharomyces cerevisiae).

  • Positive control
    • Isolated mitochondria from Human , Rat and Mouse hearts.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab110259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 10-1 µg/mg of lysate.
WB Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 30 kDa.

Target

  • Function
    Subunits I, II and III form the functional core of the enzyme complex.
  • Involvement in disease
    Defects in MT-CO3 are a cause of Leber hereditary optic neuropathy (LHON) [MIM:535000]. LHON is a maternally inherited disease resulting in acute or subacute loss of central vision, due to optic nerve dysfunction. Cardiac conduction defects and neurological defects have also been described in some patients. LHON results from primary mitochondrial DNA mutations affecting the respiratory chain complexes.
    Defects in MT-CO3 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome.
    Defects in MT-CO3 are associated with recurrent myoglobinuria mitochondrial (RM-MT) [MIM:550500]. Recurrent myoglobinuria is characterized by recurrent attacks of rhabdomyolysis (necrosis or disintegration of skeletal muscle) associated with muscle pain and weakness, and followed by excretion of myoglobin in the urine.
  • Sequence similarities
    Belongs to the cytochrome c oxidase subunit 3 family.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • COIII antibody
    • COX3 antibody
    • COX3_HUMAN antibody
    • COXIII antibody
    • Cytochrome c oxidase polypeptide III antibody
    • Cytochrome c oxidase subunit 3 antibody
    • MT CO3 antibody
    • MT-CO3 antibody
    • MTCO3 antibody
    see all

Images

  • All lanes : Anti-MTCO3 antibody [DA5BC4] (ab110259) at 4 µg/ml

    Lane 1 : Isolated mitochondria from Human heart at 5 µg
    Lane 2 : Isolated mitochondria Rat heart at 10 µg
    Lane 3 : Isolated mitochondria from Mouse heart at 10 µg

    Predicted band size: 30 kDa

References

This product has been referenced in:
  • Perks KL  et al. Adult-onset obesity is triggered by impaired mitochondrial gene expression. Sci Adv 3:e1700677 (2017). Read more (PubMed: 28835921) »
  • Taylor NG  et al. The Assembly Factor Pet117 Couples Heme a Synthase Activity to Cytochrome Oxidase Assembly. J Biol Chem 292:1815-1825 (2017). Read more (PubMed: 27998984) »
See all 25 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Question
Answer

Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Unserem Wissen nach wurde der Anti-Cytochrome C Oxidase subunit III antibody [DA5BC4] (ab110259) bisher noch nicht in IHC-Fr getestet.

Daher freue ich mich Ihnen mitteilen zu können, dass unser Abtrial für sie in Frage kommt. Falls Sie sich entscheiden unser Produkt in IHC-Fr zu testen, bekommen Sie einen Rabatt für eine zukünftige Bestellung, wenn Sie uns ein Abreview mit dem Testresultat zusenden. Der Rabatt würde den ganzen Wert von €370.00 des ab110259 abdecken, und gegen eine erneute Bestellung eines weiteren Produktes von uns verrechnet werden.

Um von diesem Angebot profitieren zu können, folgen Sie bitte den folgenden Schritten:

1.) Testen Sie den Antikörper in IHC-Fr.

2.) Teilen Sie uns das Ergebnis Ihres Tests innerhalb von 4 Monaten durch ein Abreview mit und notieren Sie den Discount Code in dem Feld "additional notes".
Unter der folgenden URL können Sie mehr über unser Abreview System erfahren:
https://www.abcam.com/abreviews

3.) Der Rabattcode ist nach dem Abschicken des Abreviews aktiv, und Sie können ein anderes Produkt zu dem gleichen Preis wie ab110259 bei uns bestellen (halten Sie bei der Bestellung bitte den Rabattcode und die Bestellnummer von ab110259 bereit). Bitte beachten Sie, dass der Rabattcode innerhalb von 4 Monaten nach Ausstellung eingelöst werden muss.

Der Rabattcode wird gültig unabhängig davon, ob Ihr Ergebnis positiv oder negativ ist.

Die Bedingungen zu unserem 100% AbTrial Rabatt können Sie unter dem folgenden Link nachlesen:
https://www.abcam.com/index.html?pageconfig=resource&rid=11998

Ihr AbTrial Code lautet: ABTRIAL-xxxxx und ist gültig bis zum 06/04/2013.

Ich hoffe, diese Informationen helfen Ihnen weiter. Falls Sie einen weitere Fragen haben, zögern Sie bitte nicht, sich wieder an mich zu wenden.

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Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of ab110259.
To check the status of the order please contact our Customer Service team and reference this number.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
I wish you the best of luck with your research.

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Answer

Vielen Dank für Ihre Geduld.

Leider war ich diese Woche krank und war daher nicht im Büro.

Ich habe Ihre Anfrage an eine Kollegin aus dem Kundendienst weitergeleitet, die Sie wegen des Bestellvorgangs kontaktieren wird.

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Answer

Vielen Dank für Ihre Anfrage.



Die gute Nachricht ist, dass wir noch drei Röhrchen der Charge GR87744 des ab110259 Anti-Cytochrome C Oxidase subunit III antibody [DA5BC4]habe. Die schlechte ist, dass sich zwei davon in unsere Zweigstelle in Tokyo befinden, und es bis zu einer Woche dauern kann, bis wir diese dann liefern können. Ich möchte daher vorschlagen, sich so schnell wie möglich bei uns zu melden, und diese zu bestellen.


Die Ziffern geben uns darüber Auskunft, um welche Abfüllung der Charge es sich handelt, bzw wann es in die Röhrchen pipettiert wurde.

Funktionieren denn alle vier Röhrchen nicht so, wie Sie es erwartet haben, oder haben Sie nur eins getestet?


Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

Benutzen Sie unsere Produkte? Schicken Sie uns einen Abreview. Verdienen Sie sich eine Belohnung!
https://www.abcam.com/abreviews

Read More

Answer

Thank you for your email.

We have reserved four vials of lot x for you. It has currently the order number x- please refer to this number when placing the order. Thank you.

I have also meanwhile heard back from the laboratory. The laboratory confirmed, that all three lots in question (including the new one you just ordered) were made from the same batch of original stock antibody. All three lots passed QC by Western blot.Mitosciences laboratory also confirmed thatthe production process has not been changed – all lots were simply diluted from stock to 1 mg/mL in HBS/0.02% azide. I am therefore at loss to konw what might has happened in your case. Please let me know what you think and if you do have another explanation which could explain the results.

Please let me know how you are getting on with the x. Please do also not hesitate to contact us, should you have any other question. I look forward to hear back from you.

Read More
Application
Western blot
Sample
Human Cell lysate - other (HeLa WT vs HeLa rho-0)
Loading amount
13 µg
Specification
HeLa WT vs HeLa rho-0
Gel Running Conditions
Reduced Denaturing (Reduced Denaturing (4-15%))
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Jul 27 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - other (Mouse Liver Mitochondria)
Loading amount
20 µg
Specification
Mouse Liver Mitochondria
Gel Running Conditions
Reduced Denaturing (10% Bis Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 01 2011

Answer

Thank you for contacting Abcam. I will be glad to help you with your inquiry for a positive control for your antibodies. We do have several mitochondrial control lysates, which I have listed below: Mouse mitochondrial liver lysate, ab110343 Mouse mitochondrial brain lysate, ab110345 Mouse mitochondrial heart lysate, ab110350 Also, if you could give me some more information on the protocol that you have used and the tissue samples, I could try and help resolve the western blot problems. If there is anything else I can help you with, please

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