• Product name
    Anti-MTCO3 antibody [DA5BC4]
  • Description
    Mouse monoclonal [DA5BC4] to MTCO3
  • Host species
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human, Saccharomyces cerevisiae
  • Immunogen

    Full length native Cytochrome C Oxidase subunit III (Saccharomyces cerevisiae).

  • Positive control
    • Isolated mitochondria from Human , Rat and Mouse hearts.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.



Our Abpromise guarantee covers the use of ab110259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 10-1 µg/mg of lysate.
WB Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 30 kDa.


  • Function
    Subunits I, II and III form the functional core of the enzyme complex.
  • Involvement in disease
    Defects in MT-CO3 are a cause of Leber hereditary optic neuropathy (LHON) [MIM:535000]. LHON is a maternally inherited disease resulting in acute or subacute loss of central vision, due to optic nerve dysfunction. Cardiac conduction defects and neurological defects have also been described in some patients. LHON results from primary mitochondrial DNA mutations affecting the respiratory chain complexes.
    Defects in MT-CO3 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome.
    Defects in MT-CO3 are associated with recurrent myoglobinuria mitochondrial (RM-MT) [MIM:550500]. Recurrent myoglobinuria is characterized by recurrent attacks of rhabdomyolysis (necrosis or disintegration of skeletal muscle) associated with muscle pain and weakness, and followed by excretion of myoglobin in the urine.
  • Sequence similarities
    Belongs to the cytochrome c oxidase subunit 3 family.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • COIII antibody
    • COX3 antibody
    • COX3_HUMAN antibody
    • COXIII antibody
    • Cytochrome c oxidase polypeptide III antibody
    • Cytochrome c oxidase subunit 3 antibody
    • MT CO3 antibody
    • MT-CO3 antibody
    • MTCO3 antibody
    see all


  • All lanes : Anti-MTCO3 antibody [DA5BC4] (ab110259) at 4 µg/ml

    Lane 1 : Isolated mitochondria from Human heart at 5 µg
    Lane 2 : Isolated mitochondria Rat heart at 10 µg
    Lane 3 : Isolated mitochondria from Mouse heart at 10 µg

    Predicted band size: 30 kDa


This product has been referenced in:
  • Perks KL  et al. Adult-onset obesity is triggered by impaired mitochondrial gene expression. Sci Adv 3:e1700677 (2017). Read more (PubMed: 28835921) »
  • Zhao C  et al. Geniposide Protects Primary Cortical Neurons against Oligomeric Aß1-42-Induced Neurotoxicity through a Mitochondrial Pathway. PLoS One 11:e0152551 (2016). WB ; Mouse . Read more (PubMed: 27046221) »

See all 24 Publications for this product

Customer reviews and Q&As

Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Unserem Wissen nach wurde der Anti-Cytochrome C Oxidase subunit III antibody [DA5BC4] (ab110259) bisher noch nicht in IHC-Fr getestet.

Daher freue ich mich Ihnen ...

Read More

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has c...

Read More

Vielen Dank für Ihre Geduld.

Leider war ich diese Woche krank und war daher nicht im Büro.

Ich habe Ihre Anfrage an eine Kollegin aus dem Kundendienst weitergeleitet, die Sie wegen des Bestellvorgangs kontaktieren wird.

Vielen Dank für Ihre Anfrage.

Die gute Nachricht ist, dass wir noch drei Röhrchen der Charge GR87744 des ab110259 Anti-Cytochrome C Oxidase subunit III antibody [DA5BC4]habe. Die schlechte ist, dass sich zwei davon in unser...

Read More

Thank you for your email.

We have reserved four vials of lot x for you. It has currently the order number x- please refer to this number when placing the order. Thank you.

I have also meanwhile heard back from the laboratory. The la...

Read More
Western blot
Human Cell lysate - other (HeLa WT vs HeLa rho-0)
Loading amount
13 µg
HeLa WT vs HeLa rho-0
Gel Running Conditions
Reduced Denaturing (Reduced Denaturing (4-15%))
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Jul 27 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Mouse Cell lysate - other (Mouse Liver Mitochondria)
Loading amount
20 µg
Mouse Liver Mitochondria
Gel Running Conditions
Reduced Denaturing (10% Bis Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 01 2011

Thank you for contacting Abcam. I will be glad to help you with your inquiry for a positive control for your antibodies. We do have several mitochondrial control lysates, which I have listed below: Mouse mitochondrial liver lysate...

Read More


Sign up