Overview

  • Product name

    Anti-MTCO3 antibody [DA5BC4]
  • Description

    Mouse monoclonal [DA5BC4] to MTCO3
  • Host species

    Mouse
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human, Saccharomyces cerevisiae
  • Immunogen

    Full length native protein (purified). This information is considered to be commercially sensitive.

  • Positive control

    • Isolated mitochondria from Human , Rat and Mouse hearts.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab110259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 10-1 µg/mg of lysate.
WB Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 30 kDa.

Target

  • Function

    Subunits I, II and III form the functional core of the enzyme complex.
  • Involvement in disease

    Defects in MT-CO3 are a cause of Leber hereditary optic neuropathy (LHON) [MIM:535000]. LHON is a maternally inherited disease resulting in acute or subacute loss of central vision, due to optic nerve dysfunction. Cardiac conduction defects and neurological defects have also been described in some patients. LHON results from primary mitochondrial DNA mutations affecting the respiratory chain complexes.
    Defects in MT-CO3 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome.
    Defects in MT-CO3 are associated with recurrent myoglobinuria mitochondrial (RM-MT) [MIM:550500]. Recurrent myoglobinuria is characterized by recurrent attacks of rhabdomyolysis (necrosis or disintegration of skeletal muscle) associated with muscle pain and weakness, and followed by excretion of myoglobin in the urine.
  • Sequence similarities

    Belongs to the cytochrome c oxidase subunit 3 family.
  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • COIII antibody
    • COX3 antibody
    • COX3_HUMAN antibody
    • COXIII antibody
    • Cytochrome c oxidase polypeptide III antibody
    • Cytochrome c oxidase subunit 3 antibody
    • MT CO3 antibody
    • MT-CO3 antibody
    • MTCO3 antibody
    see all

Images

  • All lanes : Anti-MTCO3 antibody [DA5BC4] (ab110259) at 4 µg/ml

    Lane 1 : Isolated mitochondria from Human heart at 5 µg
    Lane 2 : Isolated mitochondria Rat heart at 10 µg
    Lane 3 : Isolated mitochondria from Mouse heart at 10 µg

    Predicted band size: 30 kDa

References

This product has been referenced in:

  • Signes A  et al. APOPT1/COA8 assists COX assembly and is oppositely regulated by UPS and ROS. EMBO Mol Med 11:N/A (2019). Read more (PubMed: 30552096) »
  • Andreazza S  et al. Mitochondrially-targeted APOBEC1 is a potent mtDNA mutator affecting mitochondrial function and organismal fitness in Drosophila. Nat Commun 10:3280 (2019). Read more (PubMed: 31337756) »
See all 28 Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews

Application
Western blot
Sample
Mouse Cell lysate - other (Mitochondrial fraction of striatal ST-Hdh-Q7/Q7 ce)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris gradient gel. Laemmli buffer with 10% β-Mercaptoethanol. Samples were denatured at 55C for 10 min. Immobilon-FL transfer membranes. Overnight transfer at +4C.)
Loading amount
20 µg
Specification
Mitochondrial fraction of striatal ST-Hdh-Q7/Q7 ce
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Oct 19 2019

Application
Western blot
Sample
Human Cell lysate - other (HeLa WT vs HeLa rho-0)
Loading amount
13 µg
Specification
HeLa WT vs HeLa rho-0
Gel Running Conditions
Reduced Denaturing (Reduced Denaturing (4-15%))
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Jul 27 2012

Application
Western blot
Sample
Mouse Cell lysate - other (Mouse Liver Mitochondria)
Loading amount
20 µg
Specification
Mouse Liver Mitochondria
Gel Running Conditions
Reduced Denaturing (10% Bis Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 01 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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