Western blot abreview for Anti-MTCO3 antibody [DA5BC4]

Poor
Abreviews
Application
Western blot
Sample
Human Cell lysate - other (HeLa WT vs HeLa rho-0)
Loading amount
13 µg
Specification
HeLa WT vs HeLa rho-0
Gel Running Conditions
Reduced Denaturing (Reduced Denaturing (4-15%))
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Dilution
1/250
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST 5% milk

Secondary antibody

Name
Non-Abcam antibody was used: Santa Cruz sc-2054 mouse/human adsorbed
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000

Detection

Detection method
Pierce West Dura
Exposure
10 minute(s) and 0 second(s)
Positive control
Mitochondrial lysate of HeLa WT cells
Negative control
Mitochondrial lysate of HeLa rho-0 cells. These cells do not have mitochondrial DNA

Additional data

Additional Notes
PVDF membrane (Pierce), lysis buffer 10 mM Tris, pH=8.0, 0.5% SDS, 1x protease inhibitor cocktail Pierce PI-87875. Protein determination: BCA (Pierce)
Abcam response
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Submitted Jul 27 2012

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