Anti-MTGR1 antibody (ab128164)
Key features and details
- Rabbit polyclonal to MTGR1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MTGR1 antibody
See all MTGR1 primary antibodies -
Description
Rabbit polyclonal to MTGR1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 554-604 of Human MTGR1 (NP_005084.1).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab128164 was affinity purified using an epitope specific to MTGR1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab128164 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 67 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| Notes |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 67 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
May function as a complex with the chimeric protein RUNX1/AML1-CBFA2T1/MTG8 which is produced in acute myeloid leukemia with the chromosomal translocation t(8;21). May thus be involved in the repression of AML1-dependent transcription and the induction of G-CSF/CSF3-dependent cell growth. May be a tumor suppressor gene candidate involved in myeloid tumors with the deletion of the 20q11 region. -
Tissue specificity
Ubiquitously expressed in fetal and adult tissues. Highly expressed in adult brain, heart, lung, kidney, lymph node, appendix, thymus, testis, uterus, small intestine, prostate and thymus. -
Sequence similarities
Belongs to the CBFA2T family.
Contains 1 MYND-type zinc finger.
Contains 1 TAFH (NHR1) domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 419155 Chicken
- Entrez Gene: 9139 Human
- Omim: 601308 Human
- Omim: 603672 Human
- SwissProt: 22987 Chicken
- SwissProt: O43439 Human
- Unigene: 153934 Human
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Alternative names
- AML1 MTG8 antibody
- cbfa2t2 antibody
- Core binding factor runt domain alpha subunit 2 translocated to 2 antibody
see all
Images
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Western blot - Anti-MTGR1 antibody (ab128164)All lanes : Anti-MTGR1 antibody (ab128164) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : Jurkat whole cell lysate at 50 µg
Lane 4 : HeLa whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 67 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-MTGR1 antibody (ab128164)Detection of MTGR1 by Western Blot of Immunprecipitate.
ab128164 at 1 µg/ml staining MTGR1 in 293T whole cell lysate immunoprecipitated using ab128164 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: control IgG. Predicted band size : 67 kDa.
Detection: Chemiluminescence with exposure time of 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab128164 has been referenced in 2 publications.
- Engqvist H et al. Validation of Novel Prognostic Biomarkers for Early-Stage Clear-Cell, Endometrioid and Mucinous Ovarian Carcinomas Using Immunohistochemistry. Front Oncol 10:162 (2020). PubMed: 32133296
- Chen DC et al. CBFA2T2 is associated with a cancer stem cell state in renal cell carcinoma. Cancer Cell Int 17:103 (2017). PubMed: 29162985