Anti-MTGR1 antibody (ab128164)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-MTGR1 antibody
See all MTGR1 primary antibodies -
Description
Rabbit polyclonal to MTGR1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 554-604 of Human MTGR1 (NP_005084.1).
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Positive control
- 293T, Jurkat and HeLa whole cell lysates. IHC-P: FFPE human prostate tissue sections.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab128164 was affinity purified using an epitope specific to MTGR1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab128164 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 67 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. | |
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
May function as a complex with the chimeric protein RUNX1/AML1-CBFA2T1/MTG8 which is produced in acute myeloid leukemia with the chromosomal translocation t(8;21). May thus be involved in the repression of AML1-dependent transcription and the induction of G-CSF/CSF3-dependent cell growth. May be a tumor suppressor gene candidate involved in myeloid tumors with the deletion of the 20q11 region. -
Tissue specificity
Ubiquitously expressed in fetal and adult tissues. Highly expressed in adult brain, heart, lung, kidney, lymph node, appendix, thymus, testis, uterus, small intestine, prostate and thymus. -
Sequence similarities
Belongs to the CBFA2T family.
Contains 1 MYND-type zinc finger.
Contains 1 TAFH (NHR1) domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 419155 Chicken
- Entrez Gene: 9139 Human
- Omim: 601308 Human
- Omim: 603672 Human
- SwissProt: 22987 Chicken
- SwissProt: O43439 Human
- Unigene: 153934 Human
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Alternative names
- AML1 MTG8 antibody
- cbfa2t2 antibody
- Core binding factor runt domain alpha subunit 2 translocated to 2 antibody
see all
Images
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Western blot - Anti-MTGR1 antibody (ab128164)All lanes : Anti-MTGR1 antibody (ab128164) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : Jurkat whole cell lysate at 50 µg
Lane 4 : HeLa whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 67 kDa
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTGR1 antibody (ab128164)IHC image of MTGR1 staining in human prostate formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab128164, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Immunoprecipitation - Anti-MTGR1 antibody (ab128164)Detection of MTGR1 by Western Blot of Immunprecipitate.
ab128164 at 1 µg/ml staining MTGR1 in 293T whole cell lysate immunoprecipitated using ab128164 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: control IgG. Predicted band size : 67 kDa.
Detection: Chemiluminescence with exposure time of 30 seconds.
Datasheets and documents
References
This product has been referenced in:
- Chen DC et al. CBFA2T2 is associated with a cancer stem cell state in renal cell carcinoma. Cancer Cell Int 17:103 (2017). Read more (PubMed: 29162985) »
