Product nameMTH1 overexpression 293T lysate (whole cell)
ab94036 is a 293T cell transfected lysate in which Human MTH1 has been transiently over-expressed using a pCMV-MTH1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details on how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
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BackgroundFunction: Antimutagenic. Acts as a sanitizing enzyme for oxidized nucleotide pools, thus suppressing cell dysfunction and death induced by oxidative stress. Hydrolyzes 8-oxo-dGTP, 8-oxo-dATP and 2-OH-dATP, thus preventing misincorporation of oxidized purine nucleoside triphosphates into DNA and subsequently preventing A:T to C:G and G:C to T:A transversions. Able to hydrolyze also the corresponding ribonucleotides, 2-OH-ATP, 8-oxo-GTP and 8-oxo-ATP. Does not play a role in U8 snoRNA decapping activity. Binds U8 snoRNA. Tissue specificity: Widely expressed with highest expression in thymus, testis, embryo and proliferating blood lymphocytes. Similarity: Belongs to the Nudix hydrolase family. Contains 1 nudix hydrolase domain. Developmental stage: In peripheral blood lymphocytes, expressed at much higher levels in proliferating cells than in resting cells. PTM: The N-terminus is blocked.
Our Abpromise guarantee covers the use of ab94036 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94036 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-MTH1 antibody (ab55527) at 1/500 dilution
Lane 1 :
MTH1 overexpression 293T lysate (whole cell) (ab94036)
Lane 2 : 293T non-transfected Lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94036 has not yet been referenced specifically in any publications.