Overview

  • Product name
  • Description
    Rabbit polyclonal to MTHFD1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human MTHFD1 aa 541-571 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P11586

  • Positive control
    • Y79 cell lysate; Human lung carcinoma tissue; 293 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab174237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/10 - 1/50.
WB 1/50 - 1/100. Predicted molecular weight: 102 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Tissue specificity
    Ubiquitous.
  • Pathway
    One-carbon metabolism; tetrahydrofolate interconversion.
  • Involvement in disease
    Defects in MTHFD1 may be a cause of susceptibility to folate-sensitive neural tube defects (folate-sensitive NTD) [MIM:601634]. The most common NTDs are open spina bifida (myelomeningocele) and anencephaly. Genetic defects in MTHFD1 may affect the risk of spina bifida via the maternal rather than the embryonic genotype.
    Genetic variation in MTHFD1 could be associated with susceptibility to colorectal cancer (CRC) [MIM:114500].
  • Sequence similarities
    In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.
    In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family.
  • Domain
    This trifunctional enzyme consists of two major domains: an N-terminal part containing the methylene-THF dehydrogenase and cyclohydrolase activities and a larger C-terminal part containing formyl-THF synthetase activity.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5,10 methylenetetrahydrofolate dehydrogenase 5,10 methylenetetrahydrofolate cyclohydrolase,10 formyltetrahydrofolate synthetase antibody
    • C 1 tetrahydrofolate synthase cytoplasmic antibody
    • C1 tetrahydrofolate synthase cytoplasmic antibody
    • C1 THF synthase antibody
    • C1-THF synthase antibody
    • C1TC antibody
    • C1TC_HUMAN antibody
    • Cytoplasmic C 1 tetrahydrofolate synthase antibody
    • Formyltetrahydrofolate synthetase antibody
    • Methenyltetrahydrofolate cyclohydrolase antibody
    • Methylenetetrahydrofolate dehydrogenase (NADP+ dependent) methenyltetrahydrofolate cyclohydrolase formyltetrahydrofolate synthetase antibody
    • Methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 methenyltetrahydrofolate cyclohydrolase formyltetrahydrofolate synthetase antibody
    • Methylenetetrahydrofolate dehydrogenase antibody
    • MTHFC antibody
    • MTHFD antibody
    • MTHFD1 antibody
    • NADP dependent cyclohydrolase formyltetrahydrofolate synthetase antibody
    • OTTHUMP00000180657 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung carcinoma tissue labeling MTHFD1 with ab174237 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining. 

  • Anti-MTHFD1 antibody (ab174237) at 1/50 dilution + Y79 cell lysate at 35 µg

    Predicted band size: 102 kDa

  • Confocal immunofluorescent analysis of 293 cells labeling MTHFD1 with ab174237 at 1/10 dilution followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

References

ab174237 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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