Overview

  • Product name

  • Description

    Rabbit polyclonal to MTHFD1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Rabbit, Horse, Cow, Cat, Dog, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan, Giant Panda
  • Immunogen

    Synthetic peptide within Human MTHFD1 aa 150-200. The exact sequence is proprietary.
    Database link: P11586

  • Positive control

    • WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab226341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 102 kDa.

Target

  • Tissue specificity

    Ubiquitous.
  • Pathway

    One-carbon metabolism; tetrahydrofolate interconversion.
  • Involvement in disease

    Defects in MTHFD1 may be a cause of susceptibility to folate-sensitive neural tube defects (folate-sensitive NTD) [MIM:601634]. The most common NTDs are open spina bifida (myelomeningocele) and anencephaly. Genetic defects in MTHFD1 may affect the risk of spina bifida via the maternal rather than the embryonic genotype.
    Genetic variation in MTHFD1 could be associated with susceptibility to colorectal cancer (CRC) [MIM:114500].
  • Sequence similarities

    In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.
    In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family.
  • Domain

    This trifunctional enzyme consists of two major domains: an N-terminal part containing the methylene-THF dehydrogenase and cyclohydrolase activities and a larger C-terminal part containing formyl-THF synthetase activity.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • 5,10 methylenetetrahydrofolate dehydrogenase 5,10 methylenetetrahydrofolate cyclohydrolase,10 formyltetrahydrofolate synthetase antibody
    • C 1 tetrahydrofolate synthase cytoplasmic antibody
    • C1 tetrahydrofolate synthase cytoplasmic antibody
    • C1 THF synthase antibody
    • C1-THF synthase antibody
    • C1TC antibody
    • C1TC_HUMAN antibody
    • Cytoplasmic C 1 tetrahydrofolate synthase antibody
    • Formyltetrahydrofolate synthetase antibody
    • Methenyltetrahydrofolate cyclohydrolase antibody
    • Methylenetetrahydrofolate dehydrogenase (NADP+ dependent) methenyltetrahydrofolate cyclohydrolase formyltetrahydrofolate synthetase antibody
    • Methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 methenyltetrahydrofolate cyclohydrolase formyltetrahydrofolate synthetase antibody
    • Methylenetetrahydrofolate dehydrogenase antibody
    • MTHFC antibody
    • MTHFD antibody
    • MTHFD1 antibody
    • NADP dependent cyclohydrolase formyltetrahydrofolate synthetase antibody
    • OTTHUMP00000180657 antibody
    see all

Images

  • All lanes : Anti-MTHFD1 antibody (ab226341) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
    Lane 5 : NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 102 kDa


    Exposure time: 10 seconds


    Cell lysates were prepared using NETN lysis buffer.

  • MTHFD1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226341 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226341 at 1 µg/ml.

    Lane 1: ab226341 IP in HEK-293T whole cell lysate.

    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection: Chemiluminescence with exposure time of 10 seconds.

References

ab226341 has not yet been referenced specifically in any publications.

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