Anti-MTHFD1L antibody (ab194466)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-MTHFD1L antibody
See all MTHFD1L primary antibodies -
Description
Mouse polyclonal to MTHFD1L -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow
-
Immunogen
Recombinant fragment (GST-tag) corresponding to Human MTHFD1L aa 801-899. (NP_056255).
Sequence:VFKTDTRAEIDLVCELAKRAGAFDAVPCYHWSVGGKGSVDLARAVREAAS KRSRFQFLYDVQVPIVDKIRTIAQAVYGAKDIELSPEAQAKIDRYTQQG
Database link: Q6UB35 -
Positive control
- 293 cell lysate.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: 50% Glycerol -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab194466 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 106 kDa. |
Target
-
Function
May provide the missing metabolic reaction required to link the mitochondria and the cytoplasm in the mammalian model of one-carbon folate metabolism in embryonic an transformed cells complementing thus the enzymatic activities of MTHFD2. -
Tissue specificity
Detected in most tissues, highest expression found in placenta, thymus and brain. Low expression is found in liver and skeletal muscle. Up-regulated in colon adenocarcinoma. -
Pathway
One-carbon metabolism; tetrahydrofolate interconversion. -
Sequence similarities
In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.
In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family. -
Domain
This monofunctional enzyme consists of two major domains: an N-terminal inactive methylene-THF dehydrogenase and cyclohydrolase domain and an active larger formyl-THF synthetase C-terminal domain. -
Cellular localization
Mitochondrion. - Information by UniProt
-
Database links
- Entrez Gene: 534296 Cow
- Entrez Gene: 25902 Human
- Entrez Gene: 270685 Mouse
- Omim: 611427 Human
- SwissProt: Q0VCR7 Cow
- SwissProt: Q6UB35 Human
- SwissProt: Q3V3R1 Mouse
- Unigene: 591343 Human
see all -
Alternative names
- 10-formyl-THF synthetase antibody
- C1TM_HUMAN antibody
- Formyltetrahydrofolate synthetase antibody
see all
Images
-
Western blot - Anti-MTHFD1L antibody (ab194466)Anti-MTHFD1L antibody (ab194466) at 1/500 dilution + 293 cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 106 kDa -
Western blot - Anti-MTHFD1L antibody (ab194466)Anti-MTHFD1L antibody (ab194466) at 1/1000 dilution + recombinant Human MTHFD1L (immunogen) at 0.2 µg
Developed using the ECL technique.
Predicted band size: 106 kDaPredicted MW of immunogen: 37 KDa
Protocols
Datasheets and documents
References
ab194466 has not yet been referenced specifically in any publications.
