Key features and details
- Rabbit polyclonal to MTHFD1L - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MTHFD1L antibody - C-terminal
See all MTHFD1L primary antibodies
DescriptionRabbit polyclonal to MTHFD1L - C-terminal
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow
Recombinant fragment corresponding to Human MTHFD1L aa 759-978 (C terminal).
GVPLKKEYTEENIQLVADGCCNLQKQIQITQLFGVPVVVALNVFKTDTRA EIDLVCELAKRAGAFDAVPCYHWSVGGKGSVDLARAVREAASKRSRFQFL YDVQVPIVDKIRTIAQAVYGAKDIELSPEAQAKIDRYTQQGFGNLPICMA KTHLSLSHQPDKKGVPRDFILPISDVRASIGAGFIYPLVGTMSTMPGLPT RPCFYDIDLDTETEQVKGLF
Database link: Q6UB35
- WB: Colo320, HepG2 and HeLa whole cell lysates. IHC-P: Human colon cancer and kidney tissues.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229708 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 106 kDa.|
|IHC-P||1/20 - 1/200.|
FunctionMay provide the missing metabolic reaction required to link the mitochondria and the cytoplasm in the mammalian model of one-carbon folate metabolism in embryonic an transformed cells complementing thus the enzymatic activities of MTHFD2.
Tissue specificityDetected in most tissues, highest expression found in placenta, thymus and brain. Low expression is found in liver and skeletal muscle. Up-regulated in colon adenocarcinoma.
PathwayOne-carbon metabolism; tetrahydrofolate interconversion.
Sequence similaritiesIn the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.
In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family.
DomainThis monofunctional enzyme consists of two major domains: an N-terminal inactive methylene-THF dehydrogenase and cyclohydrolase domain and an active larger formyl-THF synthetase C-terminal domain.
- Information by UniProt
- 10-formyl-THF synthetase antibody
- C1TM_HUMAN antibody
- Formyltetrahydrofolate synthetase antibody
All lanes : Anti-MTHFD1L antibody - C-terminal (ab229708) at 1/1000 dilution
Lane 1 : Colo3320 whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
All lanes : Goat anti-rabbit IgG at 1/10000 dilution
Predicted band size: 106 kDa
Observed band size: 106 kDa
Paraffin-embedded human colon cancer tissue stained for MTHFD1L using ab229708 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human kidney tissue stained for MTHFD1L using ab229708 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229708 has been referenced in 1 publication.
- Xu T et al. MicroRNA-940 inhibits glioma progression by blocking mitochondrial folate metabolism through targeting of MTHFD2. Am J Cancer Res 9:250-269 (2019). PubMed: 30906627