Anti-MTHFD1L antibody - C-terminal (ab229708)
Key features and details
- Rabbit polyclonal to MTHFD1L - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MTHFD1L antibody - C-terminal
See all MTHFD1L primary antibodies -
Description
Rabbit polyclonal to MTHFD1L - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow -
Immunogen
Recombinant fragment corresponding to Human MTHFD1L aa 750 to the C-terminus (C terminal).
Database link: Q6UB35 -
Positive control
- WB: Colo320, HepG2 and HeLa whole cell lysates. IHC-P: Human colon cancer and kidney tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229708 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 106 kDa.
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IHC-P |
1/20 - 1/200.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 106 kDa. |
IHC-P
1/20 - 1/200. |
Target
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Function
May provide the missing metabolic reaction required to link the mitochondria and the cytoplasm in the mammalian model of one-carbon folate metabolism in embryonic an transformed cells complementing thus the enzymatic activities of MTHFD2. -
Tissue specificity
Detected in most tissues, highest expression found in placenta, thymus and brain. Low expression is found in liver and skeletal muscle. Up-regulated in colon adenocarcinoma. -
Pathway
One-carbon metabolism; tetrahydrofolate interconversion. -
Sequence similarities
In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.
In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family. -
Domain
This monofunctional enzyme consists of two major domains: an N-terminal inactive methylene-THF dehydrogenase and cyclohydrolase domain and an active larger formyl-THF synthetase C-terminal domain. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 534296 Cow
- Entrez Gene: 25902 Human
- Entrez Gene: 270685 Mouse
- Omim: 611427 Human
- SwissProt: Q0VCR7 Cow
- SwissProt: Q6UB35 Human
- SwissProt: Q3V3R1 Mouse
- Unigene: 591343 Human
see all -
Alternative names
- 10-formyl-THF synthetase antibody
- C1TM_HUMAN antibody
- Formyltetrahydrofolate synthetase antibody
see all
Images
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All lanes : Anti-MTHFD1L antibody - C-terminal (ab229708) at 1/1000 dilution
Lane 1 : Colo3320 whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Secondary
All lanes : Goat anti-rabbit IgG at 1/10000 dilution
Predicted band size: 106 kDa
Observed band size: 106 kDa -
Paraffin-embedded human colon cancer tissue stained for MTHFD1L using ab229708 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human kidney tissue stained for MTHFD1L using ab229708 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab229708 has been referenced in 2 publications.
- Xu T et al. MicroRNA-940 inhibits glioma progression by blocking mitochondrial folate metabolism through targeting of MTHFD2. Am J Cancer Res 9:250-269 (2019). PubMed: 30906627
- Sdelci S et al. MTHFD1 interaction with BRD4 links folate metabolism to transcriptional regulation. Nat Genet 51:990-998 (2019). PubMed: 31133746