Anti-MTHFD2 antibody (ab56772)

Submit a review Q&A (1)References (5)

Overview

  • Product name

    Anti-MTHFD2 antibody
    See all MTHFD2 primary antibodies

  • Description

    Mouse monoclonal to MTHFD2

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein, corresponding to amino acids 16-345 of Human MTHFD2

  • General notes

    This product was changed from ascites to tissue culture supernatant on 24/1/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab56772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 37 kDa.
IHC-P Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

Target

  • Sequence similarities

    Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.

  • Developmental stage

    Expressed only in developing normal tissues.

  • Cellular localization

    Mitochondrion.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial antibody
    • Descriptions antibody
    • Methenyltetrahydrofolate cyclohydrolase antibody
    • methylene tetrahydrofolate dehydrogenase (NAD+ dependent) antibody
    • methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2 antibody
    • methylenetetrahydrofolate dehydrogenase (NADP+ dependent) antibody
    • methylenetetrahydrofolate dehydrogenase 2 antibody
    • MGC82516 antibody
    • MTDC_HUMAN antibody
    • MTHFD2 antibody
    • mthfd2 methylene tetrahydrofolate dehydrogenase (NAD+ dependent) antibody
    • NAD-dependent methylene tetrahydrofolate dehydrogenase antibody
    • NAD-dependent methylenetetrahydrofolate dehydrogenase antibody
    • NMDMC antibody
    see all

Images

  • Western blot - Anti-MTHFD2 antibody (ab56772)
    Western blot - Anti-MTHFD2 antibody (ab56772)

    MTHFD2 antibody (ab56772) at 1μg/lane + HepG2 cell lysate at 25μg/lane.

    This image was generated using the ascites version of the product.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTHFD2 antibody (ab56772)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTHFD2 antibody (ab56772)

    MTHFD2 antibody (ab56772) used in immunohistochemistry at 3μg/ml on formalin fixed and paraffin embedded human lymphoma tissue.

  • Flow Cytometry - Anti-MTHFD2 antibody (ab56772)
    Flow Cytometry - Anti-MTHFD2 antibody (ab56772)

    Overlay histogram showing HepG2 cells stained with ab56772 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56772, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    This image was generated using the ascites version of the product.

References

This product has been referenced in:

  • Nishimura T  et al. Cancer stem-like properties and gefitinib resistance are dependent on purine synthetic metabolism mediated by the mitochondrial enzyme MTHFD2. Oncogene 38:2464-2481 (2019). Read more (PubMed: 30532069) »
  • Green NH  et al. MTHFD2 links RNA methylation to metabolic reprogramming in renal cell carcinoma. Oncogene 38:6211-6225 (2019). Read more (PubMed: 31289360) »
See all 5 Publications for this product

Publishing research using ab56772? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

will test ab56772 in flow cytometry

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Answer

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