- Datasheet
- Specific References (2)
- Protocols
Overview
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Product nameAnti-MTHFD2 antibody
See all MTHFD2 primary antibodies -
DescriptionMouse monoclonal to MTHFD2
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Host speciesMouse
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Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
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Species reactivityReacts with: Human
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Immunogen
Recombinant full length protein, corresponding to amino acids 16-345 of Human MTHFD2
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General notes
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab176016 as a replacement.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: None
PBS, pH 7.2 -
Concentration information loading... -
PurityProtein G purified
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ClonalityMonoclonal
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IsotypeIgG2a
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Light chain typekappa
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab56772 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 37 kDa. | |
| IHC-P | Use a concentration of 3 µg/ml. | |
| Flow Cyt | Use 1µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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Target
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Sequence similaritiesBelongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.
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Developmental stageExpressed only in developing normal tissues.
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Cellular localizationMitochondrion.
- Information by UniProt
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Database links
- Entrez Gene: 10797 Human
- Entrez Gene: 441024 Human
- Omim: 604887 Human
- SwissProt: P13995 Human
- SwissProt: Q9H903 Human
- Unigene: 469030 Human
- Unigene: 721011 Human
- Unigene: 740700 Human
see all -
Alternative names
- Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial antibody
- Descriptions antibody
- Methenyltetrahydrofolate cyclohydrolase antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTHFD2 antibody (ab56772)MTHFD2 antibody (ab56772) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human lymphoma tissue. -
Western blot - Anti-MTHFD2 antibody (ab56772)MTHFD2 antibody (ab56772) at 1ug/lane + HepG2 cell lysate at 25ug/lane. -
Flow Cytometry - Anti-MTHFD2 antibody (ab56772)Overlay histogram showing HepG2 cells stained with ab56772 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56772, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
References
This product has been referenced in:
- Gustafsson Sheppard N et al. The folate-coupled enzyme MTHFD2 is a nuclear protein and promotes cell proliferation. Sci Rep 5:15029 (2015). WB . Read more (PubMed: 26461067) »
- Nilsson R et al. Metabolic enzyme expression highlights a key role for MTHFD2 and the mitochondrial folate pathway in cancer. Nat Commun 5:3128 (2014). WB ; Human . Read more (PubMed: 24451681) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"