Overview

  • Product name

  • Description

    Goat polyclonal to MTHFR
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Rabbit, Chicken, Cow, Dog, Human, Pig
  • Immunogen

    Synthetic peptide:

    C-SRPKSYIYRTQEWDE

    with a Cysteine residue linker, corresponding to internal sequence amino acids 369-383 of Human MTHFR (NP_005948.3).

  • Positive control

    • NIH3T3 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab126837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 75 kDa).

Target

  • Function

    Catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co-substrate for homocysteine remethylation to methionine.
  • Pathway

    One-carbon metabolism; tetrahydrofolate interconversion.
  • Involvement in disease

    Defects in MTHFR are the cause of methylenetetrahydrofolate reductase deficiency (MTHFRD) [MIM:236250]. MTHFRD is autosomal recessive disorder with a wide range of features including homocysteinuria, homocysteinemia [MIM:603174], developmental delay, severe mental retardation, perinatal death, psychiatric disturbances, and later-onset neurodegenerative disorders.
    Defects in MTHFR may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
    Defects in MTHFR may be a cause of susceptibility to folate-sensitive neural tube defects (folate-sensitive NTD) [MIM:601634]. The most common NTDs are open spina bifida (myelomeningocele) and anencephaly.
  • Sequence similarities

    Belongs to the methylenetetrahydrofolate reductase family.
  • Information by UniProt
  • Database links

  • Alternative names

    • 5 10 methylenetetrahydrofolate reductase (NADPH) antibody
    • 5 10 methylenetetrahydrofolate reductase antibody
    • Methylenetetrahydrofolate reductase (NAD(P)H) antibody
    • Methylenetetrahydrofolate reductase antibody
    • Methylenetetrahydrofolate reductase intermediate form antibody
    • MTHFR antibody
    • MTHR_HUMAN antibody
    see all

Images

  • Anti-MTHFR antibody (ab126837) at 0.3 µg/ml + NIH 3T3 lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 75 kDa

References

ab126837 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Application
Western blot
Sample
Rat Cell lysate - whole cell (placenta)
Gel Running Conditions
Non-reduced Denaturing (4-20%)
Loading amount
30 µg
Specification
placenta
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C

Ms. María Sánchez-Campillo

Verified customer

Submitted Sep 07 2018

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