Western blot abreview for Anti-MTHFR antibody

Below Average
Abreviews
Application
Western blot
Sample
Rat Cell lysate - whole cell (placenta)
Gel Running Conditions
Non-reduced Denaturing (4-20%)
Loading amount
30 µg
Specification
placenta
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1250

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: anti-goat IG-HRP
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 70 kDa Non-specific: 10, 15, 20, 25, 30, kDa
Positive control
Yes. beta actine

Additional data

Abcam response
We would like to thank this researcher for submitting their review. This antibody had not been tested for cross reactivity with rat and we were unsure of what the results would be. The reviewer received an AbTrial discount code for using the antibody in an untested species.

Ms. María Sánchez-Campillo

Verified customer

Submitted Sep 07 2018

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