Anti-MTHFR antibody (ab236737)
Key features and details
- Rabbit polyclonal to MTHFR
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-MTHFR antibody
See all MTHFR primary antibodies -
Description
Rabbit polyclonal to MTHFR -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human MTHFR aa 1-656.
Database link: P42898 -
Positive control
- WB: HEK-293T whole cell lysate. IHC-P: Human prostate cancer tissue. ICC/IF: HeLa cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
-
Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236737 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/20 - 1/200.
|
|
WB |
1/500 - 1/2000. Predicted molecular weight: 75 kDa.
|
|
ICC/IF |
1/50 - 1/200.
|
Notes |
---|
IHC-P
1/20 - 1/200. |
WB
1/500 - 1/2000. Predicted molecular weight: 75 kDa. |
ICC/IF
1/50 - 1/200. |
Target
-
Function
Catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co-substrate for homocysteine remethylation to methionine. -
Pathway
One-carbon metabolism; tetrahydrofolate interconversion. -
Involvement in disease
Defects in MTHFR are the cause of methylenetetrahydrofolate reductase deficiency (MTHFRD) [MIM:236250]. MTHFRD is autosomal recessive disorder with a wide range of features including homocysteinuria, homocysteinemia [MIM:603174], developmental delay, severe mental retardation, perinatal death, psychiatric disturbances, and later-onset neurodegenerative disorders.
Defects in MTHFR may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
Defects in MTHFR may be a cause of susceptibility to folate-sensitive neural tube defects (folate-sensitive NTD) [MIM:601634]. The most common NTDs are open spina bifida (myelomeningocele) and anencephaly. -
Sequence similarities
Belongs to the methylenetetrahydrofolate reductase family. - Information by UniProt
-
Database links
- Entrez Gene: 4524 Human
- Omim: 607093 Human
- SwissProt: P42898 Human
- Unigene: 214142 Human
-
Alternative names
- 5 10 methylenetetrahydrofolate reductase (NADPH) antibody
- 5 10 methylenetetrahydrofolate reductase antibody
- Methylenetetrahydrofolate reductase (NAD(P)H) antibody
see all
Images
-
Anti-MTHFR antibody (ab236737) at 1/500 dilution + HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 75 kDa -
Paraffin-embedded human prostate cancer tissue stained for MTHFR using ab236737 at 1/100 dilution in immunohistochemical analysis.
-
HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for MTHFR (green) using ab236737 at 1/100 dilution in ICC/IF. Secondary antibody is an Alexa-Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab236737 has not yet been referenced specifically in any publications.