Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab82153 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.5 - 1.5 µg/ml. Predicted molecular weight: 23 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionContributes to tetrahydrofolate metabolism. Helps regulate carbon flow through the folate-dependent one-carbon metabolic network that supplies carbon for the biosynthesis of purines, thymidine and amino acids. Catalyzes the irreversible conversion of 5-formyltetrahydrofolate (5-FTHF) to yield 5,10-methenyltetrahydrofolate.
Sequence similaritiesBelongs to the 5-formyltetrahydrofolate cyclo-ligase family.
- Information by UniProt
- 10-methenyl-tetrahydrofolate synthetase antibody
- 5 10 methenyl tetrahydrofolate synthetase antibody
- 5 antibody
Anti-MTHFS antibody (ab82153) at 0.5 µg/ml + human liver lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 23 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Exposure time: 1 hour
ab82153 has not yet been referenced specifically in any publications.